Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)
Key features and details
- Goat Anti-Rat IgG H&L (HRP) preadsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Dot blot
Overview
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Product name
Goat Anti-Rat IgG H&L (HRP) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rat -
Tested applications
Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Dot blotmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Sheep more details -
Immunogen
Full length native Rat IgG (purified).
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Conjugation notes
Horseradish Peroxidase (HRP) -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/500 - 1/1500.
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IHC-P |
Use at an assay dependent dilution.
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IHC-Fr |
Use at an assay dependent dilution.
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|
WB |
Use at an assay dependent dilution.
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ELISA |
1/50000.
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|
Dot blot |
Use at an assay dependent dilution.
|
Notes |
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ICC/IF
1/500 - 1/1500. |
IHC-P
Use at an assay dependent dilution. |
IHC-Fr
Use at an assay dependent dilution. |
WB
Use at an assay dependent dilution. |
ELISA
1/50000. |
Dot blot
Use at an assay dependent dilution. |
Images
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Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097) at 1/1000 dilution + Rat IgG, 50 ng
Both the heavy and light chains of rat IgG are detected.
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IHC - Wholemount - Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)This picture was kindly supplied as part of the review submitted by Helen Makarenkova.
Whole mount immunostaining of E11.5 mouse embryo (Blood vessels in the embryonic head). This staining was performed with rat monoclonal antibody to Pecam followed by staining with ab7097 (HRP conjugated).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (32)
ab7097 has been referenced in 32 publications.
- Wu M et al. Plant DNA polymerases a and d mediate replication of geminiviruses. Nat Commun 12:2780 (2021). PubMed: 33986276
- Liu G et al. CDK6 is stimulated by hyperthermia and protects gastric cancer cells from hyperthermia-induced damage. Mol Med Rep 23:N/A (2021). PubMed: 33537819
- Li W et al. MiR-27a-3p suppresses cerebral ischemia-reperfusion injury by targeting FOXO1. Aging (Albany NY) 13:11727-11737 (2021). PubMed: 33875617
- Liu Y et al. Proteomic Analysis Revealed the Characteristics of Key Proteins Involved in the Regulation of Inflammatory Response, Leukocyte Transendothelial Migration, Phagocytosis, and Immune Process during Early Lung Blast Injury. Oxid Med Cell Longev 2021:8899274 (2021). PubMed: 34007409
- Paulino DSM et al. Diacerein treatment prevents colitis-associated cancer in mice. World J Clin Oncol 11:732-746 (2020). PubMed: 33033695