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  1. Link

    goat-rat-igg-hl-hrp-preadsorbed-ab7097.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (32)

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Western blot - Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)
  • IHC - Wholemount - Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)

Key features and details

  • Goat Anti-Rat IgG H&L (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Dot blot

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Overview

  • Product name

    Goat Anti-Rat IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Rat
  • Tested applications

    Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ELISA, Dot blotmore details
  • Minimal
    cross-reactivity


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Sheep
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • Immunogen

    Full length native Rat IgG (purified).

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes

    Horseradish Peroxidase (HRP)
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Rat
    • IgG
    • Enzyme
    • HRP

Associated products

  • Substrate reagent

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/500 - 1/1500.
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution.
ELISA
1/50000.
Dot blot
Use at an assay dependent dilution.
Notes
ICC/IF
1/500 - 1/1500.
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution.
ELISA
1/50000.
Dot blot
Use at an assay dependent dilution.

Images

  • Western blot - Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)
    Western blot - Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)
    Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097) at 1/1000 dilution + Rat IgG, 50 ng


    Both the heavy and light chains of rat IgG are detected.

  • IHC - Wholemount - Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)
    IHC - Wholemount - Goat Anti-Rat IgG H&L (HRP) preadsorbed (ab7097)This picture was kindly supplied as part of the review submitted by Helen Makarenkova.

    Whole mount immunostaining of E11.5 mouse embryo (Blood vessels in the embryonic head). This staining was performed with rat monoclonal antibody to Pecam followed by staining with ab7097 (HRP conjugated).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (32)

Publishing research using ab7097? Please let us know so that we can cite the reference in this datasheet.

ab7097 has been referenced in 32 publications.

  • Wu M  et al. Plant DNA polymerases a and d mediate replication of geminiviruses. Nat Commun 12:2780 (2021). PubMed: 33986276
  • Liu G  et al. CDK6 is stimulated by hyperthermia and protects gastric cancer cells from hyperthermia-induced damage. Mol Med Rep 23:N/A (2021). PubMed: 33537819
  • Li W  et al. MiR-27a-3p suppresses cerebral ischemia-reperfusion injury by targeting FOXO1. Aging (Albany NY) 13:11727-11737 (2021). PubMed: 33875617
  • Liu Y  et al. Proteomic Analysis Revealed the Characteristics of Key Proteins Involved in the Regulation of Inflammatory Response, Leukocyte Transendothelial Migration, Phagocytosis, and Immune Process during Early Lung Blast Injury. Oxid Med Cell Longev 2021:8899274 (2021). PubMed: 34007409
  • Paulino DSM  et al. Diacerein treatment prevents colitis-associated cancer in mice. World J Clin Oncol 11:732-746 (2020). PubMed: 33033695
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

Please see customers inquiry below:

I bought Primary and secondary antibodies from sapphire biosciences almost two years ago. Upon arrival I immediately aliqoute into smaller volume and kept them in -80 degree ever since. Do you think they are still okay to use?

Primary Ab: F4/80 monoclonal antibody; Cat#AB6640 (Abcam)

Secondary Ab: Goat polyclonal secondary antibody to Rat IgG-H &L (HRP), pre-adsorbed; Cat#AB7097 (Abcam)




Customer Service & Technical Support | mailto:julic@sapphirebioscience.com

126 Cope Street │ Waterloo NSW 2017 │ Australia

Ph: 1800 062 088│ Fax: +61 2 9698 1022 | http://www.sapphirebioscience.com/

Read More

Abcam community

Verified customer

Asked on Jan 17 2013

Answer

Thank you for contacting us.

Please reffer the customer to our FAQs and our Storage guide:

"For many of our antibodies, freezing at -20oC or -80oC in small aliquots is the optimal storage condition. Aliquotting minimizes damage due to freezing and thawing, as well as contamination introduced by pipetting from a single vial multiple times. Aliquots are to be frozen and thawed once, with any remainder kept at 4oC. Upon receiving the antibody, centrifuge at 10,000 x g for 20 seconds to pull down solution that is trapped in the threads of the vial, and transfer aliquots into low-protein-binding microcentrifuge tubes. The size of the aliquots will depend on how much one typically uses in an experiment. Aliquots should be no smaller than 10 µl; the smaller the aliquot, the more the stock concentration is affected by evaporation and adsorption of the antibody onto the surface of the storage vial.


Enzyme-conjugated antibodies, should not be frozen at all and should instead be kept at 4oC. Freezing and thawing will reduce enzymatic activity in addition to affecting the antibody binding capacity.

However, when the HRP conjugated abs wasn't thought in between, the perfomance should still be ok.

The rule of the thump is that a -80C stored antibody should be fine for the next 10 years.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Jan 17 2013

Question

We received a recommendation for a secondary antibody that is inappropriate for our experiment (cross-reactive with mouse IgG). Please send a secondary that is cross-absorbed to remove reactivity with mouse IgG.

Read More

Abcam community

Verified customer

Asked on Sep 16 2011

Answer

As requested, I have issued a free of charge replacement for ab7097, anti-rat IgG cross-absorbed to remove reactivity with mouse IgG. I apologize for the misunderstanding on our part and wish you the best of luck with your research.

Read More

Abcam Scientific Support

Answered on Sep 16 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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