Product nameGoat anti-Rat IgG H&L (PE) Pre-Adsorbed
See all IgG secondary antibodies
DescriptionGoat anti-Rat IgG H&L (PE) Pre-Adsorbed
Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details
Cow, Horse, Human, Mouse, Rabbit, SheepTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Full length native protein (purified) corresponding to IgG.
ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPrepared from monospecific antiserum by immunoaffinity chromatography using rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
Conjugation notesR-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Our Abpromise guarantee covers the use of ab7010 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|Immunomicroscopy||1/50 - 1/200.|
|Flow Cyt||1/100 - 1/250.
Use 1 x 106 cells in flow cytometry is approximately 1.0 µg of antibody conjugate.
This product has been referenced in:
- Cao Y et al. Isoflurane-induced postoperative cognitive dysfunction is mediated by hypoxia-inducible factor-1a-dependent neuroinflammation in aged rats. Mol Med Rep 17:7730-7736 (2018). Read more (PubMed: 29620198) »
- Lei X et al. Diazoxide inhibits of ER stress-mediated apoptosis during oxygen-glucose deprivation in vitro and cerebral ischemia-reperfusion in vivo. Mol Med Rep 17:8039-8046 (2018). Read more (PubMed: 29693708) »