Key features and details
- Goat polyclonal Secondary Antibody to Rat IgM - mu chain, pre-adsorbed
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Product nameGoat Anti-Rat IgM mu chain preadsorbed
See all IgM secondary antibodies
DescriptionGoat polyclonal Secondary Antibody to Rat IgM - mu chain, pre-adsorbed
SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgM. Cross reactivity with IgA and IgG is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and mouse IgM was detected.
Tested applicationsSuitable for: ICC, IHC-P, ELISA, WBmore details
Human, MouseTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Our Abpromise guarantee covers the use of ab98365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent dilution.|
|IHC-P||1/200 - 1/2000.|
|ELISA||1/100 - 1/30000. Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000|
|WB||1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab98365 has been referenced in 1 publication.
- Yuasa H et al. Effects of the expansion of bacterial colonies into the intervillous spaces on the localization of several lymphocyte lineages in the rat ileum. J Vet Med Sci 81:555-566 (2019). PubMed: 30799326