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Immunology Immunoglobulins Heavy Chain IgG
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Goat Anti-Syrian hamster IgG H&L (Alexa Fluor® 488) (ab180063)

  • Datasheet
Submit a review Submit a question References (3)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Syrian hamster IgG H&L (Alexa Fluor® 488) (ab180063)
  • Alexa Fluor® - Goat Anti-Syrian hamster IgG H&L (Alexa Fluor® 488) (ab180063)

Key features and details

  • Goat Anti-Syrian hamster IgG H&L (Alexa Fluor® 488)
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: IHC-P, ELISA, ICC/IF, Flow Cyt, IHC-Fr

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Overview

  • Product name

    Goat Anti-Syrian hamster IgG H&L (Alexa Fluor® 488)
    See all IgG secondary antibodies
  • Host species

    Goat
  • Target species

    Syrian hamster
  • Specificity

    This antibody is specific to Syrian Hamster IgG
  • Tested applications

    Suitable for: IHC-P, ELISA, ICC/IF, Flow Cyt, IHC-Frmore details
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Hamster
    • IgG
    • Fluorophore
    • Alexa Fluor® 488

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab180063 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    ELISA
    Use at an assay dependent concentration.
    ICC/IF
    1/200 - 1/1000.
    Flow Cyt
    1/2000.
    IHC-Fr
    Use at an assay dependent concentration.
    Notes
    IHC-P
    1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    ELISA
    Use at an assay dependent concentration.
    ICC/IF
    1/200 - 1/1000.
    Flow Cyt
    1/2000.
    IHC-Fr
    Use at an assay dependent concentration.

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Syrian hamster IgG H&L (Alexa Fluor® 488) (ab180063)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Syrian hamster IgG H&L (Alexa Fluor® 488) (ab180063)

      IHC image of Podoplanin / gp36 staining in a section of formalin-fixed paraffin-embedded normal mouse skin. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6). Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab11936 at 1/250. The secondary antibody (shown in green) was ab180063 at 1/500 (Goat Anti-Syrian hamster IgG H&L (Alexa Fluor® 488) and counterstained using ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

      The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    • Alexa Fluor® - Goat Anti-Syrian hamster IgG H&L (Alexa Fluor® 488) (ab180063)
      Alexa Fluor® - Goat Anti-Syrian hamster IgG H&L (Alexa Fluor® 488) (ab180063)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab180063? Please let us know so that we can cite the reference in this datasheet.

    ab180063 has been referenced in 3 publications.

    • Nguyen TM  et al. The proportion of alveolar type 1 cells decreases in murine hypoplastic congenital diaphragmatic hernia lungs. PLoS One 14:e0214793 (2019). PubMed: 30995255
    • Lang JF  et al. Standard screening methods underreport AAV-mediated transduction and gene editing. Nat Commun 10:3415 (2019). PubMed: 31363095
    • Inoue T  et al. Upregulation of sphingosine-1-phosphate receptor 3 on fibroblast-like synoviocytes is associated with the development of collagen-induced arthritis via increased interleukin-6 production. PLoS One 14:e0218090 (2019). PubMed: 31173610

    Customer reviews and Q&As

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