Goat Anti-Syrian Hamster IgG H&L (HRP) (ab6892)

Submit a review Q&A (2)References (4)

Key features and details

  • Goat Anti-Syrian Hamster IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Goat
  • Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF

Overview

  • Product name

    Goat Anti-Syrian Hamster IgG H&L (HRP)
    See all IgG secondary antibodies

  • Host species

    Goat

  • Target species

    Syrian hamster

  • Tested applications

    Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IFmore details

  • Immunogen

    Full length native hamster IgG (purified).

  • Conjugation

    HRP

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.424% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...

  • Purity

    Affinity purified

  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • Conjugation notes

    Horseradish Peroxidase (HRP)

  • Clonality

    Polyclonal

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
Use at an assay dependent dilution.
ELISA
1/7000 - 1/35000.
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent dilution.
WB
1/1000 - 1/5000.
ICC/IF
1/500 - 1/2500.
Notes
Dot blot
Use at an assay dependent dilution.
ELISA
1/7000 - 1/35000.
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent dilution.
WB
1/1000 - 1/5000.
ICC/IF
1/500 - 1/2500.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (4)

Publishing research using ab6892? Please let us know so that we can cite the reference in this datasheet.

ab6892 has been referenced in 4 publications.

  • Walpita P  et al. A VLP-based vaccine provides complete protection against Nipah virus challenge following multiple-dose or single-dose vaccination schedules in a hamster model. NPJ Vaccines 2:21 (2017). PubMed: 29263876
  • Sharma G  et al. Purification and characterization of adenovirus core protein VII: a histone-like protein that is critical for adenovirus core formation. J Gen Virol 98:1785-1794 (2017). PubMed: 28695796
  • Huang Y  et al. Identification of novel genes associated with renal tertiary lymphoid organ formation in aging mice. PLoS One 9:e91850 (2014). PubMed: 24637805
  • Hoffhines AJ  et al. Tyrosylprotein sulfotransferase-2 expression is required for sulfation of RNase 9 and Mfge8 in vivo. J Biol Chem 284:3096-105 (2009). PubMed: 19047058

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

We have bought the following conjugates from your company. We asked the agancy that we bought about the temperature to keep them to increase the time of keeping. They told us to keep them in -20 C0 and we did for two weeks up to now, but now when we checked your website information it is recommended to be kept in 4 degree castigates.

Please let me know if keeping them in -20 C0 has made any problem for them and can we continue to keep them in this temperature or not?

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Best,

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Answer

Thank you for contacting us.

The datasheet of these antibodies say "Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles"

This mean after receiving the antibody you can keep antibody at 4C for 1-2 weeks when using in your experiments. After you finish your experiment we recomendaliquoting the antibody 5-10 ul in vialsand storing at -20C. We suggest this to avoidthe extra freeze thawing steps, every time you use the antibody.

If you have already stored it at -20C, this is absolutely fine!

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

I would like to find out which species, Armenian or Syrian, of hamster　the Goat anti-Hamster IgG HRP (product code ab6892) is from. I could not　find this information on the data sheet provided. Thank you,

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Answer

Thank you for your enquiry. I can confirm that the immunogen used for this product is Golden Syrian Hamster IgG. Please do not hesitate to contact me should you need any further information.

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