Anti-GODZ/DHHC-3 antibody (ab31837)
Key features and details
- Rabbit polyclonal to GODZ/DHHC-3
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GODZ/DHHC-3 antibody -
Description
Rabbit polyclonal to GODZ/DHHC-3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Xenopus laevis -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab31837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration. PubMed: 19001095
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
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Notes |
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IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. PubMed: 19001095 |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). |
Target
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Function
Palmitoyltransferase with broad specificity. Palmitoylates GABA receptors on their gamma subunit (GABRG1, GABRG2 and GABRG3), which regulates synaptic clustering and/or cell surface stability. Palmitoylates glutamate receptors GRIA1 and GRIA2, which leads to their retention in Golgi. -
Sequence similarities
Belongs to the DHHC palmitoyltransferase family.
Contains 1 DHHC-type zinc finger. -
Domain
The DHHC domain is required for palmitoyltransferase activity. -
Post-translational
modificationsAutopalmitoylated. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 51304 Human
- Entrez Gene: 69035 Mouse
- Entrez Gene: 301081 Rat
- SwissProt: Q9NYG2 Human
- SwissProt: Q8R173 Mouse
- Unigene: 61430 Human
- Unigene: 28300 Mouse
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Alternative names
- DHHC-3 antibody
- GABA-A receptor-associated membrane protein 1 antibody
- GODZ antibody
see all
Images
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Anti-GODZ/DHHC-3 antibody (ab31837) at 1 µg/ml + Cerebellum (Mouse) Tissue Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab31837 has been referenced in 10 publications.
- Yao H et al. A peptidic inhibitor for PD-1 palmitoylation targets its expression and functions. RSC Chem Biol 2:192-205 (2021). PubMed: 34458782
- Kutchukian C et al. NPC1 regulates the distribution of phosphatidylinositol 4-kinases at Golgi and lysosomal membranes. EMBO J 40:e105990 (2021). PubMed: 34019311
- Mukherjee A et al. Specific Palmitoyltransferases Associate with and Activate the Epithelial Sodium Channel. J Biol Chem 292:4152-4163 (2017). PubMed: 28154191
- Kilpatrick CL et al. Dissociation of Golgi-associated DHHC-type Zinc Finger Protein (GODZ)- and Sertoli Cell Gene with a Zinc Finger Domain-ß (SERZ-ß)-mediated Palmitoylation by Loss of Function Analyses in Knock-out Mice. J Biol Chem 291:27371-27386 (2016). PubMed: 27875292
- Lievens PM et al. ZDHHC3 Tyrosine Phosphorylation Regulates Neural Cell Adhesion Molecule Palmitoylation. Mol Cell Biol 36:2208-25 (2016). WB ; Mouse . PubMed: 27247265
- Lai J & Linder ME Oligomerization of DHHC protein S-acyltransferases. J Biol Chem 288:22862-70 (2013). PubMed: 23793055
- Levy AD et al. Subcellular Golgi localization of stathmin family proteins is promoted by a specific set of DHHC palmitoyl transferases. Mol Biol Cell 22:1930-42 (2011). PubMed: 21471001
- Shmueli A et al. Ndel1 palmitoylation: a new mean to regulate cytoplasmic dynein activity. EMBO J 29:107-19 (2010). WB, ICC/IF ; Human . PubMed: 19927128
- Noritake J et al. Mobile DHHC palmitoylating enzyme mediates activity-sensitive synaptic targeting of PSD-95. J Cell Biol 186:147-60 (2009). WB, ICC/IF ; Rat . PubMed: 19596852
- Tsutsumi R et al. Identification of G protein alpha subunit-palmitoylating enzyme. Mol Cell Biol 29:435-47 (2009). WB, ICC/IF ; Human . PubMed: 19001095