Overview

  • Product name

    Anti-GODZ/DHHC-3 antibody
  • Description

    Rabbit polyclonal to GODZ/DHHC-3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Mouse GODZ/DHHC-3 aa 250 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab31882, ab31883)

  • Positive control

    • Mouse Cerebellum Tissue Lysate
  • General notes

    Previously labelled as GODZ.

Properties

Applications

Our Abpromise guarantee covers the use of ab31837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 19001095
IHC-P 1/75. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

Target

  • Function

    Palmitoyltransferase with broad specificity. Palmitoylates GABA receptors on their gamma subunit (GABRG1, GABRG2 and GABRG3), which regulates synaptic clustering and/or cell surface stability. Palmitoylates glutamate receptors GRIA1 and GRIA2, which leads to their retention in Golgi.
  • Sequence similarities

    Belongs to the DHHC palmitoyltransferase family.
    Contains 1 DHHC-type zinc finger.
  • Domain

    The DHHC domain is required for palmitoyltransferase activity.
  • Post-translational
    modifications

    Autopalmitoylated.
  • Cellular localization

    Golgi apparatus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • DHHC-3 antibody
    • GABA-A receptor-associated membrane protein 1 antibody
    • GODZ antibody
    • Golgi-specific DHHC zinc finger protein antibody
    • Gramp1 antibody
    • Palmitoyltransferase ZDHHC3 antibody
    • Protein DHHC1 antibody
    • ZDHC3 antibody
    • ZDHC3_HUMAN antibody
    • ZDHHC3 antibody
    • Zinc finger DHHC domain-containing protein 3 antibody
    • Zinc finger protein 373 antibody
    • ZNF373 antibody
    see all

Images

  • (Image courtesy of Human Protein Atlas)
    ab31837 detecting GODZ/DHHC-3 protein in the purkinje cells of normal human cerebellum. Paraffin embedded human cerebral cortex tissue was incubated with ab31837 (1/75 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

  • Anti-GODZ/DHHC-3 antibody (ab31837) at 1 µg/ml + Cerebellum (Mouse) Tissue Lysate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 34 kDa
    Observed band size: 34 kDa
    Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute

References

This product has been referenced in:

  • Mukherjee A  et al. Specific Palmitoyltransferases Associate with and Activate the Epithelial Sodium Channel. J Biol Chem 292:4152-4163 (2017). Read more (PubMed: 28154191) »
  • Kilpatrick CL  et al. Dissociation of Golgi-associated DHHC-type Zinc Finger Protein (GODZ)- and Sertoli Cell Gene with a Zinc Finger Domain-ß (SERZ-ß)-mediated Palmitoylation by Loss of Function Analyses in Knock-out Mice. J Biol Chem 291:27371-27386 (2016). Read more (PubMed: 27875292) »
See all 8 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you for contacting us.

Here are the links to the Human Protein Atlas as well as to the mouse protein entry in UniProt database:

http://www.proteinatlas.org/ENSG00000163812/cell
http://www.proteinatlas.org/ENSG00000163812

http://www.uniprot.org/uniprot/Q8R173:
Tissue specificity in mouse: Ubiquitously expressed, with highest levels in brain. Expressed in neurons from all brain regions

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Read More
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde

Dr. Sophie Pezet

Verified customer

Submitted Sep 08 2010

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