Product nameGold-Carboxyl Conjugation Kit (40 nm, 40 OD)
40nm Gold-Carboxyl Nanoparticles (40OD) (ab269942) are coated gold nanoparticles functionalized with carboxyl groups that allow the covalent conjugation of antibodies, using the water-soluble carbodiimide crosslinker EDC.
Unlike traditional EDC/NHS coupling used to activate carboxyl groups, 40nm Gold-Carboxyl Nanoparticles (40OD) (ab269942) are optimized for single step EDC covalent coupling without signs of aggregation. This eliminates the need for EDC/NHS pre-activation and washing steps associated with traditional conjugations.
This process is also significantly faster than standard methods, with conjugates ready to use in less than 35 minutes.
40nm Gold-Carboxyl Nanoparticles (40OD) (ab269942) have a narrow size distribution, a uniform spherical shape and high batch-to-batch consistency:
Mean Diameter 36-44nm
Benefits of 40nm Gold-Carboxyl Nanoparticles (40OD):
Easy protocol – conjugate ready to use in less than 35 min, with no additional pre-activation or wash steps
Proprietary surface coating prevents metal-protein interactions, and enables covalent attachment to the gold – stable conjugates formed
Uniform spherical shape and narrow size distribution – consistent high quality and batch to batch reproducibility
Composition: 40nm carboxyl-gold 40 OD carboxyl gold nanoparticles web
Adsorption max: 530nm
Particles per ml: 36 x 1011
Volume: 1ml and 5ml
Buffer: 10mM EPPS pH 8.5
This product is manufactured by Expedeon, an Abcam company. It was previously called 40nm InnovaCoat® GOLD - Carboxyl (40 OD). Expedeon productcode 3400-0001 is the same as the 40 µg size of this product and code 3400-0005 is the same as the 200 µg size of this product.
Storage instructionsShipped at Room Temperature. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7
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Lateral Flow Assay of 40nm Gold-Carboxyl Nanoparticles (40OD) (ab269942) conjugated with EDC (1 step) to a monoclonal anti-CRP antibody titrated against CRP in buffer compared to a 2 steps EDC/NHS reaction using a competitor’s 40nm carboxyl-gold. 40nm Gold-Carboxyl Nanoparticles (40OD) (ab269942) shows a better sensitivity than the competitor, no aggregation is seen and requires only 35 minutes to make. Following the competitor’s instruction, their product required from 3 to 5 hours for the EDC/NHS activation and conjugation reaction and significant optimization to prevent aggregation.
ab269942 has not yet been referenced specifically in any publications.