Product nameGold Conjugation Kit (10nm, Maleimide)
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Gold Conjugation Kit (10 nm, Maleimide) (ab269903) provides an easy-to-use, one step procedure that allows researchers to covalently label proteins, peptides, oligonucleotides and other biomolecules containing primary amines with ultra-stable Gold nanoparticles with only 5 minutes hands-on time; furthermore conjugates are ready to use within 60 minutes.
The conjugation reacton is initiated by adding a solution of the thiol-active molecule (protein, peptide, antibody, antibody fragment or oligonucleotide) to the Gold-Maleimide nanoparticles.
Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other biomolecules to Gold nanoparticles.
This product is manufactured by Expedeon, an Abcam company, and was previously called InnovaCoat® GOLD – Maleimide Site Specific Labeling Kit (10nm). 272-0005 is the same as the 120 µl size. 272-0015 is the same as the 400 µl size.
Amount and volume of antibody for conjugation to Gold nanoparticles (10 nm, Maleimide).
amount of biomolecule
Buffer Requirements for Conjugation
Free thiols (e.g. β-mercaptoethanol, DTT) must be excluded from samples and maleimide reaction buffers by desalting, because they will compete for coupling sites on the gold nanoparticles; a desalting step post reduction is mandatory if thiols are used to reduce the disulphide bonds.
Compatible buffer constituents
The kit is compatible with PBS, MOPS, HEPES, sugars, salts and detergents.
Incompatible buffer constituents
The kit is incompatible with thiol reagents.
Only purified biomolecules are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 120 µl 400 µl Diluent reagent 1 unit 1 unit Gold mix (Lyophilized) 3 vials 1 vial Quencher reagent 1 vial 1 vial Reaction Buffer 1 vial 1 vial
ab269903 has not yet been referenced specifically in any publications.