Overview

  • Product name
    GOLD Conjugation Kit (60nm 20OD)
  • Product overview

    Abcam’s GOLD Conjugation Kit (60nm, 20OD) (ab188216) allows antibodies or proteins to be covalently attached to ultra-stable* Gold nanoparticles at very high OD quickly and easily. The hands-on time for the Gold conjugation procedure is about 2 minutes and the conjugate is ready to use within 15 minutes.

    *Gold has a protective surface coat that can withstand the most extreme conditions (e.g. 2.5M NaOH at 70°C for >1 hour).

    The Gold nanoparticles in this kit are supplied as a freeze dried mixture. The conjugation reaction is initiated simply by adding a solution of the antibody, which becomes attached (via lysine residues) to the gold surface.

    The resulting covalent conjugates are more stable than those prepared by passive adsorption methods. Moreover, unlike passive methods, the coating procedure is not dependent on the isoelectric point of the antibody, and extensive trials at different pH values are not required; all antibodies react at a single fixed pH.

  • Notes

    The 3 and 10 Test Conjugation Kits are designed to label 12 µl of antibody per vial. The 1 Test Conjugation Kit is designed to label 120 µl of antibody per vial.

  • Tested applications
    Suitable for: Conjugationmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 3 tests 10 tests 1 tests
    Gold 60nm 3 vials 10 vials 1 vial
    Gold 60nm Antibody Diluent 1 vial 1 vial 1 vial
    Gold 60nm Quencher Reagent 1 vial 1 vial 1 vial
    Gold 60nm Reaction Buffer 1 vial 1 vial 1 vial
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab188216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Conjugation Use at an assay dependent concentration.

Images

Protocols

References

This product has been referenced in:
  • Tvrdonova M  et al. Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry. Anal Bioanal Chem 411:559-564 (2019). Read more (PubMed: 30109381) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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