Product nameGOLD Conjugation Kit (80nm, 20 OD)
This product is manufactured by Expedeon, an Abcam company. Expedeon product code 231-0010 was previously called 80nm InnovaCoat® GOLD 10 Reaction 80nm Mini Kit and is the same as the 10 tests size of this product. Expedeon product code 231-0015 was previously called 80nm InnovaCoat® GOLD 1 Reaction 80nm Midi Kit and is the same as the 1 test size of this product. Expedeon product code 231-0005 was previously called 80nm InnovaCoat® GOLD 3 reaction 80nm Mini Kit and is the same as the 3 tests size of this product.
Abcam's Gold Conjugation Kit allows antibodies or proteins to be covalently attached to ultra-stable GOLD nanoparticles at very high OD quickly and easily. The hands-on time for the Gold Conjugation procedure is about 2 minutes and the conjugate is ready to use within 20 minutes.
The Gold nanoparticles in this kit are supplied as a freeze dried mixture. The conjugation reaction is initiated simply by adding a solution of the antibody, which becomes attached (via lysine residues) to the Gold surface.
The resulting covalent conjugates are more stable than those prepared by passive adsorption methods. Moreover, unlike passive methods, the coating procedure is not dependent on the isoelectric point of the antibody, and extensive trials at different pH values are not required; all antibodies react at a single fixed pH.
The 3 and 10 Test Conjugation Kits are designed to label 12 µl per vial.
The 1 Test Conjugation Kit is designed to label 120 µl per vial.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 3 tests 10 tests 1 tests Gold 3 vials 10 vials 1 vial Gold Antibody Diluent 1 vial 1 vial 1 vial Gold Quencher Reagent 1 vial 1 vial 1 vial Gold Reaction Buffer 1 vial 1 vial 1 vial
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