Question (72956) | GOLD Conjugation Kit (80nm, 20 OD) (ab154876)

Go to datasheet (ab154876)

Question

I have some questions about the Gold-biotin Easylink kits.
1) Is the biotin already conjugated to the gold?
2) Can I dissolve this in PBS with serum? Or is serum not advised?
3) I have used a smaller gold particle for my lateral flow assay before and seen low signal to noise ratio, does a larger size particle improve this?

Answer

Thank you for contacting us.

We have never tested the Biotin-GOLD with serum (PBS is fine) but they should be stable thanks to the coating.Yes, we have experienced in our LFA that bigger nanoparticles have an improved signal to noise ratio.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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