Overview

  • Product name
    GOLD-Hydrazide Conjugation Kit (40nm, 10OD)
  • Product overview

    Antibodies can be covalently attached quickly and easily, in a site-specific fashion, to ultra stable GOLD nanoparticles functionalized with hydrazides (1 min hands-on time and 30 min incubation).

     

    While antibodies in their native state do not contain aldehyde groups, it is easy to introduce aldehydes by mild oxidation of the sugar chains located in the Fc domain. Covalent attachment of aldehyde-modified antibodies to hydrazide surfaces orients the two antigen-binding arms in space, which can lead to enhanced assay performance.

     

    GOLD-Hydrazide Conjugation Kit (40 nm, 10OD) (ab188284) provides all the materials required to conjugate three antibodies in site specific manner to GOLD. Sufficient material is provided for small scale trial conjugations and then scale up of the best reagent.

     

  • Tested applications
    Suitable for: Conjugationmore details

Properties

  • Storage instructions
    Please refer to protocols.
  • Components 1 tests 3 tests
    GOLD-Hydrazide Antibody Oxidation Reagent 1 vial 3 vials
    GOLD-Hydrazide 1 x 1.5ml 1 x 4ml
    GOLD-Hydrazide Antibody Diluent 1 x 500µl 1 x 500µl
    GOLD-Hydrazide Wash/Coupling Buffer 1 x 20ml 1 x 50ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab188284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Conjugation Use at an assay dependent concentration.

Protocols

References

ab188284 has not yet been referenced specifically in any publications.

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