Product nameGOLD mouse antibody purification kit
This product is manufactured by Expedeon, an Abcam company. Expedeon product code 261-0005 was previously called AbPure™ Mouse Specific Purification System 1 purification and is the same as the 1 test size of this product. Expedeon product code 261-0010 was previously called AbPure™ Mouse Specific Purification System 3 purification and is the same as the 3 tests size of this product.
GOLD mouse antibody purification kit (ab204910) removes contaminants often found in commercial antibodies (e.g. BSA, glycine, tris and azide) that interfere in conjugation reactions with GOLD particles using our GOLD conjugation kits. It can also be used to purify mouse antibodies from crude samples such as ascites fluid. The purification system can be used with antibody volumes from 100-500 μL. 20 to 150 μg of antibody can be purified in each test.
The method involves capturing the mouse antibody on Mouse Resin and removing unwanted substances by a simple wash procedure, which is performed in a standard microfuge. The purified antibody is then eluted and neutralized.
Antibodies purified using the GOLD mouse antibody purification kit are fully compatible with our GOLD antibody conjugation kits. To purify antibodies for use with our other conjugation kits, we recommend our Mouse Antibody Purification Kit ab128745.
(1) The GOLD mouse antibody purification kit is not suitable for use with antibodies from other species.
(2) The binding strength of bovine IgG to the Mouse Resin is negligible.
(3) ab204910 is not suitable for use with antibodies from species other than mouse.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 tests 3 tests 10X Binding Buffer 1 vial 1 vial Additional Collecting Tubes 4 units 12 units Elution Buffer 1 vial 1 vial Mouse Resin 1 vial 3 vials Neutralizer 1 vial 1 vial Spin Cartridges/ Collection tubes assemblies 1 unit 3 units Wash Buffer 1 vial 1 vial
Our Abpromise guarantee covers the use of ab204910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|AP||Use at an assay dependent concentration.|
ab204910 has not yet been referenced specifically in any publications.