GOLD-Streptavidin Conjugation Reagent (40nm 10 OD) (ab186864)

Overview

  • Product name
    GOLD-Streptavidin Conjugation Reagent (40nm 10 OD)
  • Product overview

    Abcam's GOLD-Streptavidin Conjugation Reagent (40nm 10 OD) is a unique coated nanoparticle that has an avidly bound protective surface coat which can withstand the most extreme conditions (e.g. 2.5M NaOH at 70oC for ~2 hours).

    By covalently attaching streptavidin to the surface coat the streptavidin ligand is irreversibly attached and cannot desorb, which is a major problem with products in which the streptavidin is passively adsorbed to the metal.
    GOLD-Streptavidin binds with high affinity to biotinylated molecules.

    A common application for Streptavidin GOLD is to be used as a detector in Lateral Flow Assays (LFAs).

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 200 µl 1 ml
    Gold-Streptavidin 1 x 200µl 1 x 1ml

Images

  • The 3 possibilities of binding are shown in the image:

    A   Ideal concentration of Biotin conjugated Rabbit IgG (50-100 μg/ml) binds to the Streptavidin GOLD producing a strong signal on the test line (Goat anti-rabbit IgG).

    B   Excess Biotin conjugated Rabbit IgG (>400 μg/ml) results in competition between the unbound Biotin conjugated Rabbit IgG and Streptavidin GOLD for the test line.

    C   A lack of Biotin conjugated Rabbit IgG (<10 μg/ml) leads to a weak test line.

Protocols

References

ab186864 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer


1. What is the streptavidin concentration? Is it approximately 3mg/mL?
We have never tested exactly how many streptavidin particles there are per gold nanoparticle however I can tell you the number of gold nanoparticles coated in streptavidin within the vial is 1.8 x10^11.

2.
Unfortunately we don't know how many binding sites there are available on the Streptavidin because of the way they coat the surface of the gold nanoparticles, however it is worth noting that because the biotin-streptavidin bond is so strong it should only take one biotin to bind the streptavidin.

3.
We have never tried using our GOLD-streptavidin conjugation reagent in mass cytometry. However if other similar products are used for this application, I am not aware of any reason why our product could not be used.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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