Product nameGOLD-Streptavidin Conjugation Reagent (40nm 10 OD)
This product is manufactured by Expedeon, an Abcam company. Expedeon product code 250-1000 was previously called 40nm InnovaCoat® GOLD (10OD) Streptavidin Conjµgate 1 ml and is the same as the 1 ml size of this product. Expedeon product code 250-0200 was previously called 40nm InnovaCoat® GOLD (10OD) Streptavidin Conjµgate 0.2 ml and is the same as the 200 µl size of this product.
Abcam's GOLD-Streptavidin Conjugation Reagent (40nm 10 OD) is a unique coated nanoparticle that has an avidly bound protective surface coat which can withstand the most extreme conditions (e.g. 2.5M NaOH at 70oC for ~2 hours).
By covalently attaching streptavidin to the surface coat the streptavidin ligand is irreversibly attached and cannot desorb, which is a major problem with products in which the streptavidin is passively adsorbed to the metal.
GOLD-Streptavidin binds with high affinity to biotinylated molecules.
A common application for Streptavidin GOLD is to be used as a detector in Lateral Flow Assays (LFAs).
Storage instructionsStore at +4°C. Please refer to protocols.
Components 200 µl 1 ml Gold-Streptavidin 1 x 200µl 1 x 1ml
The 3 possibilities of binding are shown in the image:
A Ideal concentration of Biotin conjugated Rabbit IgG (50-100 μg/ml) binds to the Streptavidin GOLD producing a strong signal on the test line (Goat anti-rabbit IgG).
B Excess Biotin conjugated Rabbit IgG (>400 μg/ml) results in competition between the unbound Biotin conjugated Rabbit IgG and Streptavidin GOLD for the test line.
C A lack of Biotin conjugated Rabbit IgG (<10 μg/ml) leads to a weak test line.
ab186864 has not yet been referenced specifically in any publications.