Question (76449) | GOLD-Streptavidin Conjugation Reagent (40nm 10 OD) (ab186864)

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Question


1. What is the streptavidin concentration? Is it approximately 3mg/mL?

2. Do you know approximately how many biotin sites are free for each streptavidin?

3. Is there any reason (that you know of) that this reagent could NOT be used for mass cytometry (i.e. CyTOF)?

Answer


1. What is the streptavidin concentration? Is it approximately 3mg/mL?
We have never tested exactly how many streptavidin particles there are per gold nanoparticle however I can tell you the number of gold nanoparticles coated in streptavidin within the vial is 1.8 x10^11.

2.
Unfortunately we don't know how many binding sites there are available on the Streptavidin because of the way they coat the surface of the gold nanoparticles, however it is worth noting that because the biotin-streptavidin bond is so strong it should only take one biotin to bind the streptavidin.

3.
We have never tried using our GOLD-streptavidin conjugation reagent in mass cytometry. However if other similar products are used for this application, I am not aware of any reason why our product could not be used.

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