Anti-GOLGA3 antibody (ab96080)
Key features and details
- Rabbit polyclonal to GOLGA3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-GOLGA3 antibody
See all GOLGA3 primary antibodies -
Description
Rabbit polyclonal to GOLGA3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 1436 and 1498 of GOLGA3 (NP_005886).
-
Positive control
- 293T, A431, H1299 cell lysates for WB and HeLa cells for IF
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 167 kDa.
|
|
ICC/IF |
1/100 - 1/200.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 167 kDa. |
ICC/IF
1/100 - 1/200. |
Target
-
Function
Golgi auto-antigen; probably involved in maintaining Golgi structure. -
Tissue specificity
Expressed in all tissues tested. Expressed in liver, testis, lung, heart, salivary gland and kidney. -
Domain
Extended rod-like protein with coiled-coil domains. -
Post-translational
modificationsCleaved by caspases in apoptotic cells. -
Cellular localization
Cytoplasm. Golgi apparatus, Golgi stack membrane. - Information by UniProt
-
Database links
- Entrez Gene: 2802 Human
- Omim: 602581 Human
- SwissProt: Q08378 Human
- Unigene: 507333 Human
-
Alternative names
- GCP170 antibody
- GOGA3_HUMAN antibody
- GOLGA 3 antibody
see all
Images
-
All lanes : Anti-GOLGA3 antibody (ab96080) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 167 kDa
5% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96080 at 1/200 dilution. Lower image merged with DNA probe.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab96080 has been referenced in 3 publications.
- Yue X et al. Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner. Commun Biol 4:1370 (2021). PubMed: 34876695
- Tang CX et al. Golgin-160 and GMAP210 play an important role in U251 cells migration and invasion initiated by GDNF. PLoS One 14:e0211501 (2019). PubMed: 30695072
- Turowec JP et al. An unbiased proteomic screen reveals caspase cleavage is positively and negatively regulated by substrate phosphorylation. Mol Cell Proteomics 13:1184-97 (2014). WB ; Human . PubMed: 24556848