Product nameAnti-GOLGA3 antibody
See all GOLGA3 primary antibodies
DescriptionRabbit polyclonal to GOLGA3
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide, corresponding to a region within amino acids 1436 and 1498 of GOLGA3 (NP_005886).
- 293T, A431, H1299 cell lysates for WB and HeLa cells for IF
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 167 kDa.|
|ICC/IF||1/100 - 1/200.|
RelevanceGOLGA3 is a member of the golgin family of proteins which are localized to the Golgi. It has been postulated to play a role in nuclear transport and Golgi apparatus localization.
Cellular localizationCytoplasmic and Golgi Apparatus
- GCP170 antibody
- GOLGA 3 antibody
- GOLGA3 antibody
All lanes : Anti-GOLGA3 antibody (ab96080) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 167 kDa
5% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96080 at 1/200 dilution. Lower image merged with DNA probe.
This product has been referenced in:
- Tang CX et al. Golgin-160 and GMAP210 play an important role in U251 cells migration and invasion initiated by GDNF. PLoS One 14:e0211501 (2019). Read more (PubMed: 30695072) »
- Turowec JP et al. An unbiased proteomic screen reveals caspase cleavage is positively and negatively regulated by substrate phosphorylation. Mol Cell Proteomics 13:1184-97 (2014). WB ; Human . Read more (PubMed: 24556848) »