Key features and details
- Rabbit polyclonal to GOLGA3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GOLGA3 antibody
See all GOLGA3 primary antibodies
DescriptionRabbit polyclonal to GOLGA3
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide, corresponding to a region within amino acids 1436 and 1498 of GOLGA3 (NP_005886).
- 293T, A431, H1299 cell lysates for WB and HeLa cells for IF
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 167 kDa.|
|ICC/IF||1/100 - 1/200.|
RelevanceGOLGA3 is a member of the golgin family of proteins which are localized to the Golgi. It has been postulated to play a role in nuclear transport and Golgi apparatus localization.
Cellular localizationCytoplasmic and Golgi Apparatus
- GCP170 antibody
- GOLGA 3 antibody
- GOLGA3 antibody
All lanes : Anti-GOLGA3 antibody (ab96080) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 167 kDa
5% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96080 at 1/200 dilution. Lower image merged with DNA probe.
ab96080 has been referenced in 2 publications.
- Tang CX et al. Golgin-160 and GMAP210 play an important role in U251 cells migration and invasion initiated by GDNF. PLoS One 14:e0211501 (2019). PubMed: 30695072
- Turowec JP et al. An unbiased proteomic screen reveals caspase cleavage is positively and negatively regulated by substrate phosphorylation. Mol Cell Proteomics 13:1184-97 (2014). WB ; Human . PubMed: 24556848