Overview

  • Product name

  • Description

    Rabbit polyclonal to GOLGA3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1436 and 1498 of GOLGA3 (NP_005886).

  • Positive control

    • 293T, A431, H1299 cell lysates for WB and HeLa cells for IF

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 167 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Relevance

    GOLGA3 is a member of the golgin family of proteins which are localized to the Golgi. It has been postulated to play a role in nuclear transport and Golgi apparatus localization.
  • Cellular localization

    Cytoplasmic and Golgi Apparatus
  • Database links

  • Alternative names

    • GCP170 antibody
    • GOLGA 3 antibody
    • GOLGA3 antibody
    • Golgi autoantigen antibody
    • Golgi complex associated protein of 170 kDa antibody
    • Golgi complex-associated protein of 170 kD antibody
    • Golgi membrane associated protein antibody
    • Golgi peripheral membrane protein antibody
    • Golgin 160 antibody
    • Golgin subfamily a 3 antibody
    • HGNC:4426 antibody
    • Male enhanced antigen 2 antibody
    • MEA2 antibody
    • SY2/SY10 protein antibody
    see all

Images

  • All lanes : Anti-GOLGA3 antibody (ab96080) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 167 kDa



    5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96080 at 1/200 dilution. Lower image merged with DNA probe.

References

This product has been referenced in:

  • Tang CX  et al. Golgin-160 and GMAP210 play an important role in U251 cells migration and invasion initiated by GDNF. PLoS One 14:e0211501 (2019). Read more (PubMed: 30695072) »
  • Turowec JP  et al. An unbiased proteomic screen reveals caspase cleavage is positively and negatively regulated by substrate phosphorylation. Mol Cell Proteomics 13:1184-97 (2014). WB ; Human . Read more (PubMed: 24556848) »
See all 2 Publications for this product

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