Anti-GOLGA5/Golgin-84 antibody (ab155806)
Key features and details
- Rabbit polyclonal to GOLGA5/Golgin-84
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-GOLGA5/Golgin-84 antibody
See all GOLGA5/Golgin-84 primary antibodies -
Description
Rabbit polyclonal to GOLGA5/Golgin-84 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human GOLGA5/Golgin-84 aa 237-566 (internal sequence).
Database link: Q8TBA6 -
Positive control
- A549 and Mouse brain whole cell lysates, methanol-fixed HeLa cells, paraffin-embedded Human smooth muscle tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155806 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 83 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use 10mM Citrate buffer or alternatively Tris-EDTA buffer (pH 8.0) may be used
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 83 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use 10mM Citrate buffer or alternatively Tris-EDTA buffer (pH 8.0) may be used |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Involved in maintaining Golgi structure. Stimulates the formation of Golgi stacks and ribbons. Involved in intra-Golgi retrograde transport. -
Tissue specificity
Ubiquitous. Highly expressed in seminiferous tubules and Leydig cells in testis, and detected at much lower levels in the other tissues tested. Expression is very low or not detectable in spermatozoa. -
Involvement in disease
Defects in GOLGA5 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving GOLGA5 is found in thyroid papillary carcinomas. Translocation t(10;14)(q11;q32) with RET. The translocation generates the RET/GOLGA5 (PTC5) oncogene which was found in 2 cases of PACT in children exposed to radioactive fallout after Chernobyl. -
Post-translational
modificationsHighly phosphorylated during mitosis. Phosphorylation is barely detectable during interphase. -
Cellular localization
Golgi apparatus membrane. Found throughout the Golgi, both on cisternae and, at higher abundance, on the tubulo-vesicular structures of the cis-Golgi network. - Information by UniProt
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Database links
- Entrez Gene: 510412 Cow
- Entrez Gene: 9950 Human
- Entrez Gene: 27277 Mouse
- Entrez Gene: 299258 Rat
- Omim: 606918 Human
- SwissProt: Q8TBA6 Human
- SwissProt: Q9QYE6 Mouse
- SwissProt: Q3ZU82 Rat
see all -
Alternative names
- Cell proliferation inducing gene 31 antibody
- Cell proliferation-inducing gene 31 protein antibody
- GOGA5_HUMAN antibody
see all
Images
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Anti-GOLGA5/Golgin-84 antibody (ab155806) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 83 kDa
7.5% SDS PAGE -
Anti-GOLGA5/Golgin-84 antibody (ab155806) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 83 kDa
7.5% SDS PAGE -
Confocal immunofluorescence analysis of methanol-fixed HeLa cells labeling GOLGA5/Golgin-84 with ab155806 at 1/500 dilution (green). Alpha-tubulin filaments were labeled with an anti-alpha-tubulin antibody at 1/2500 (red).
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Immunohistochemical analysis of paraffin-embedded Human smooth muscle tissue, labeling GOLGA5/Golgin-84 with ab155806 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155806 has not yet been referenced specifically in any publications.