Overview

  • Product name

    Anti-GOLGA5/Golgin-84 antibody
    See all GOLGA5/Golgin-84 primary antibodies
  • Description

    Mouse polyclonal to GOLGA5/Golgin-84
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human GOLGA5/Golgin-84.
    Database link: NP_005104.2

  • Positive control

    • Human liver tisssue lysate, HepG2 cell lysate, GOLGA5/Golgin-84 transfected 293T cell lysate and HeLa cells.
  • General notes

     This product was previously labelled as GOLGA5

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    Involved in maintaining Golgi structure. Stimulates the formation of Golgi stacks and ribbons. Involved in intra-Golgi retrograde transport.
  • Tissue specificity

    Ubiquitous. Highly expressed in seminiferous tubules and Leydig cells in testis, and detected at much lower levels in the other tissues tested. Expression is very low or not detectable in spermatozoa.
  • Involvement in disease

    Defects in GOLGA5 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving GOLGA5 is found in thyroid papillary carcinomas. Translocation t(10;14)(q11;q32) with RET. The translocation generates the RET/GOLGA5 (PTC5) oncogene which was found in 2 cases of PACT in children exposed to radioactive fallout after Chernobyl.
  • Post-translational
    modifications

    Highly phosphorylated during mitosis. Phosphorylation is barely detectable during interphase.
  • Cellular localization

    Golgi apparatus membrane. Found throughout the Golgi, both on cisternae and, at higher abundance, on the tubulo-vesicular structures of the cis-Golgi network.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cell proliferation inducing gene 31 antibody
    • Cell proliferation-inducing gene 31 protein antibody
    • GOGA5_HUMAN antibody
    • GOLGA 5 antibody
    • GOLGA5 antibody
    • Golgi autoantigen golgin subfamily a 5 antibody
    • Golgi integral membrane protein 5 antibody
    • Golgin 84 antibody
    • Golgin A5 antibody
    • Golgin subfamily A member 5 antibody
    • Golgin-84 antibody
    • Golgin84 antibody
    • GOLIM 5 antibody
    • GOLIM5 antibody
    • Protein Ret-II antibody
    • PTC5 antibody
    • Ret fused gene 5 antibody
    • RET fused gene 5 protein antibody
    • Ret II antibody
    • Ret II protein antibody
    • RET-fused gene 5 protein antibody
    • RFG 5 antibody
    • RFG5 antibody
    see all

Images

  • Anti-GOLGA5/Golgin-84 antibody (ab89136) at 1 µg/ml + Human liver tissue lysate at 50 µg

    Predicted band size: 83 kDa
    Observed band size: 83 kDa

  • Anti-GOLGA5/Golgin-84 antibody (ab89136) at 1 µg/ml + HepG2 cell lysate at 50 µg

    Predicted band size: 83 kDa
    Observed band size: 83 kDa

  • All lanes : Anti-GOLGA5/Golgin-84 antibody (ab89136) at 1 µg/ml

    Lane 1 : GOLGA5/Golgin-84 transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 83 kDa
    Observed band size: 83 kDa

  • ab89136 at 10µg/ml staining GOLGA5/Golgin-84 in HeLa cells by Immunofluorescence.

References

ab89136 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab89136.
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