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Golgi/ER Staining Kit - Cytopainter (ab139485)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (3) Submit a question References (1)

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Fluoroescent staining using CytoPainter Golgi/ER Staining Kit (ab139485)
  • Absorbance and fluorescent emission - blue dye
  • Fluorescence image - red dye
  • Fluorescence image - green dye
  • Fluoroescent staining using Organelle Reagent III

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Overview

  • Product name

    Golgi/ER Staining Kit - Cytopainter
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Golgi/ER Staining Kit | Cytopainter (ab139485) provides a rapid and convenient method for visualizing the Golgi and ER within living cells, without requiring lengthy transfection procedures. ab139485 is validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line Jurkat, canine kidney cell line MDCK, and human bone osteosarcoma epithelial cell line U2OS. The kit should also be suitable for identifying Golgi body and endoplasmic reticulum perturbing agents and thus can be a useful tool for examining the transport and recycling of molecules from the GA to ER in cellular secretory pathways.


    Organelle Reagent III contains a mixture of GA-selective, ER-selective and nucleus-selective dyes suitable for live cell staining. Compared with other commercially available dyes for labeling Golgi bodies, the green dye component of the Reagent III is more faithfully localized to the Golgi apparatus, with minimal staining of the endoplasmic reticulum. The red dye component of the Reagent III stains the endoplasmic reticulum with high fidelity and is specifically designed for use with green fluorescing probes.


     Review other dyes and kits for Golgi staining, or the live cell staining fluorescent dyes guide

  • Platform

    Fluorescence microscope

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 100 tests
    10X Assay Buffer 1 1 x 15ml
    50X Assay Buffer 2 1 x 1.2ml
    Organelle Reagent III (lyophilized) 1 vial
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • Cell Staining Kits
    • Endoplasmic Reticulum Staining Kits
    • Kits/ Lysates/ Other
    • Kits
    • Cell Staining Kits
    • Golgi Staining Kits

Associated products

    Images

    • Fluoroescent staining using CytoPainter Golgi/ER Staining Kit (ab139485)
      Fluoroescent staining using CytoPainter Golgi/ER Staining Kit (ab139485)This image is courtesy of an Abreview submitted by Edwin Hernandez

      CytoPainter Golgi/ER Staining Kit (ab139485) staining live rat astrocyte cells. Cells were seeded on a Ibidi u-slide 18 well flat poly-L-Lysine coated. Cells were washed with 1XAssay Solution, incubated for 30 min at 37º with the Organelle Reagent III, washed 3 times with ice cold medium (DMEM F-12) and incubated again in medium for 30 minutes at 37º.

      ER (red), Golgi (green), Nuclear (blue).

    • Absorbance and fluorescent emission - blue dye
      Absorbance and fluorescent emission - blue dye
      Absorbance and fluorescent emission spectra for the blue dye
    • Fluorescence image - red dye
      Fluorescence image - red dye
      Fluorescence excitation and emission spectra for the red dye
    • Fluorescence image - green dye
      Fluorescence image - green dye
      Fluorescence excitation and emission spectra for the green dye
    • Fluoroescent staining using Organelle Reagent III
      Fluoroescent staining using Organelle Reagent III
      ab139485 kit staining of MDCK epithelial cells. The ER (red), Golgi (green) and nuclear (blue) dyes highlight their respective subcellular targets with high dependability.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab139485? Please let us know so that we can cite the reference in this datasheet.

    ab139485 has been referenced in 1 publication.

    • Okada T & Ogura T Nanoscale imaging of untreated mammalian cells in a medium with low radiation damage using scanning electron-assisted dielectric microscopy. Sci Rep 6:29169 (2016). PubMed: 27375121

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Primary Rat Astrocytes

    Excellent Average 3/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    Live rat astrocyte stained with CytoPainter Golgi/ER Staining kit.
    Cells were seeded on a Ibidi u-slide 18 well flat poly-L-Lysine coated.
    Cells were washed with 1XAssay Solution, incubated for 30 min at 37º with the Organelle Reagent III, washed 3 times with ice cold medium (DMEM F-12) and incubated again in medium for 30 minutes at 37º.

    Mr. Edwin Hernandez

    Verified customer

    Submitted Nov 05 2013

    Live cell imaging of HeLa cells - Golgi (green), ER (red), and nucleus - blue.

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    Live cell imaging of HeLa cells - Golgi (green), ER (red), and nucleus - blue.

    Dr. Armen Petrosyan

    Verified customer

    Submitted Jul 19 2019

    murine intestinal epithelial cells

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    ModeK cells (murine small intestinal epithelial cells) were grown on glass slides and stained as recommended.

    Small intestinal organoids were stained in matrigel droplets. To avoid dissociation of the matrigel all "ice cold" steps were bperformed at 10-12 °C. Half the concentration of Organelle reagent 3 was used.

    Abcam user community

    Verified customer

    Submitted Nov 07 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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