Overview

  • Product name

  • Description

    Rabbit polyclonal to Golgin-245
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Golgin-245 aa 1482-1584.
    Database link: Q13439

  • Positive control

    • Human stomach tissue
  • General notes

     This product was previously labelled as Golgin subfamily A member 4

     

Properties

Applications

Our Abpromise guarantee covers the use of ab122250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May play role in delivery of transport vesicles containing GPI-linked proteins from the trans-Golgi network through its interaction with MACF1.
  • Sequence similarities

    Contains 1 GRIP domain.
  • Domain

    Extended rod-like protein with coiled-coil domains.
  • Cellular localization

    Cytoplasm. Golgi apparatus membrane. Golgi apparatus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 256 kDa golgin antibody
    • 72.1 protein antibody
    • GCP2 antibody
    • GOGA4_HUMAN antibody
    • GOLG antibody
    • GOLGA4 antibody
    • golgi autoantigen, golgin subfamily a, 4 antibody
    • Golgin subfamily A member 4 antibody
    • golgin-240 antibody
    • Golgin-245 antibody
    • MU-RMS-40.18 antibody
    • OTTHUMP00000161363 antibody
    • OTTHUMP00000208897 antibody
    • OTTHUMP00000209805 antibody
    • p230 antibody
    • Protein 72.1 antibody
    • Trans-Golgi p230 antibody
    see all

Images

  • Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & vesicles.
  • ab122250, at 1/1200, staining Golgin-245 in paraffin embedded Human stomach tissue by Immunohistochemistry.

References

ab122250 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mice Hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Mice Hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Apr 04 2019

Application
Western blot
Sample
Mouse Cell lysate - other (Golgi membrane fraction from mice liver)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
30 µg
Specification
Golgi membrane fraction from mice liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Feb 14 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HepG2 cells)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
HepG2 cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Dec 21 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Dec 21 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2 cells)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
25 µg
Specification
HepG2 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Dec 12 2016

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