Overview

  • Product name

  • Description

    Mouse polyclonal to Golgin 97
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-767 of Human Golgin 97 (AAH32853.1).

  • Positive control

    • Golgin 97 transfected 293T cell lysate; Human pancreas tissue lysate; HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.20
    Constituent: 100% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 88 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    Probably involved in maintaining Golgi structure.
  • Sequence similarities

    Contains 1 GRIP domain.
  • Domain

    Extended rod-like protein with coiled-coil domains.
  • Cellular localization

    Golgi apparatus > Golgi stack membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Gap junction protein alpha 4 37kD antibody
    • GOGA1_HUMAN antibody
    • Golga 1 antibody
    • GOLGA1 antibody
    • Golgi autoantigen golgin subfamily a 1 antibody
    • Golgi autoantigen golgin subfamily A member 1 antibody
    • Golgi autoantigen golgin subfamily a1. antibody
    • Golgin A1 antibody
    • Golgin subfamily A member 1 antibody
    • Golgin-97 antibody
    • Golgin97 antibody
    • MGC33154 antibody
    see all

Images

  • Anti-Golgin 97 antibody (ab169287) at 1 µg/ml + Human pancreas tissue lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 88 kDa

  • Immunofluorescent analysis of HeLa cells labeling Golgin 97 with ab169287 at 10 µg/ml.
  • All lanes : Anti-Golgin 97 antibody (ab169287) at 1 µg/ml

    Lane 1 : Golgin 97 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 88 kDa

References

This product has been referenced in:

  • Kovacs L  et al. Gorab is a Golgi protein required for structure and duplication of Drosophila centrioles. Nat Genet 50:1021-1031 (2018). Read more (PubMed: 29892014) »
See 1 Publication for this product

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