• Product name

  • Description

    Rabbit polyclonal to Golgin 97
  • Host species

  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Golgin 97 aa 750 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab34458)

  • Positive control

    • ICC/IF: HepG2, MCF7 and hCMEC/D3 cells. IHC-P: Human breast carcinoma tissue.
  • General notes

    ab84340 is raised using the same immunogen as ab33701. Batches sold under ab84340 have passed QC testing in ICC/IF and IHC. In our hands, this product did not work in WB. For further information please contact our Customer Services team.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab84340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • Function

    Probably involved in maintaining Golgi structure.
  • Sequence similarities

    Contains 1 GRIP domain.
  • Domain

    Extended rod-like protein with coiled-coil domains.
  • Cellular localization

    Golgi apparatus > Golgi stack membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Gap junction protein alpha 4 37kD antibody
    • GOGA1_HUMAN antibody
    • Golga 1 antibody
    • GOLGA1 antibody
    • Golgi autoantigen golgin subfamily a 1 antibody
    • Golgi autoantigen golgin subfamily A member 1 antibody
    • Golgi autoantigen golgin subfamily a1. antibody
    • Golgin A1 antibody
    • Golgin subfamily A member 1 antibody
    • Golgin-97 antibody
    • Golgin97 antibody
    • MGC33154 antibody
    see all


  • ICC/IF image of ab84340 stained human HepG2 cells. The cells were methanol fixed (5 min), permeabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab84340, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • IHC image of Golgin 97 staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84340, 5µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ab84340 staining Golgin 97 in murine testis tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using 0.01M sodium citrate. Samples were then blocked using 10%FCS, 0.5% BSA, 1% Triton X-100, 88.5% PBS X1 and then incubated with ab84340 at a 1/100 dilution for 10 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated goat anti-rabbit IgG used at a 1/200 dilution.

    See Abreview

  • ab84340 staining Golgin-97 in MCF7 cells treated with brefeldin A (ab120299), by ICC/IF. Increase in Golgin-97 expression correlates with increased concentration of brefeldin A, as described in literature.
    The cells were incubated at 37øC for 1.5 h in media containing different concentrations of ab120299 (brefeldin A) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab84340 (5 µg/ml) was performed overnight at 4øC in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • hCMEC/D3 cells stained for Golgin 97 (green) using ab84340 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG.

    See Abreview


This product has been referenced in:

  • Zulkefli KL  et al. A role for Rab11 in the homeostasis of the endosome-lysosomal pathway. Exp Cell Res 380:55-68 (2019). Read more (PubMed: 30981667) »
  • Hsu RM  et al. Golgi tethering factor golgin-97 suppresses breast cancer cell invasiveness by modulating NF-?B activity. Cell Commun Signal 16:19 (2018). Read more (PubMed: 29703230) »
See all 11 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Human Cell (RWPE-1 - prosate epithelial cells)
Yes - 0.2% Triton X-100
RWPE-1 - prosate epithelial cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Jul 19 2019

Western blot
Human Cell lysate - whole cell (22Rv1 - prostate cancer cells)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
20 µg
22Rv1 - prostate cancer cells
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Jul 18 2019

Immunocytochemistry/ Immunofluorescence
Human Cell (hCMEC/D3 endothelial cell line)
Yes - 0.3% Triton
hCMEC/D3 endothelial cell line
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Nov 28 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M sodium citrate
Blocking step
10%FCS 0.5% BSA 1% Triton X-100 88.5% PBSX1 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 25°C

Mr. Yoav Elkis

Verified customer

Submitted Jan 04 2012

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