- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-GOLPH2 antibody
See all GOLPH2 primary antibodies -
DescriptionRabbit polyclonal to GOLPH2
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Host speciesRabbit
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Specificityab92612 will not recognize GOLPH1 or GOLPH3.
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Tested applicationsSuitable for: ICC/IF, WB, ELISA, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic 18 amino acid peptide from near the center of Human GOLPH2 (GenBank accession no. EAW62705).
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Positive control
- Rat brain tissue lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab92612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 20 µg/ml. | |
| WB | Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 45 kDa. | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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FunctionUnknown. Cellular response protein to viral infection.
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Tissue specificityWidely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), it is strongly expressed in hepatocyte-derived syncitial giant cells. Constitutively expressed by biliary epithelial cells but not by hepatocytes.
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Sequence similaritiesBelongs to the GOLM1/CASC4 family.
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Post-translational
modificationsGlycosylated.
Phosphorylation sites are present in the extracelllular medium. -
Cellular localizationGolgi apparatus > cis-Golgi network membrane. Early Golgi. Cycles via the cell surface and endosomes upon lumenal pH disruption.
- Information by UniProt
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Database links
- Entrez Gene: 51280 Human
- Entrez Gene: 105348 Mouse
- Entrez Gene: 680692 Rat
- Omim: 606804 Human
- SwissProt: Q8NBJ4 Human
- SwissProt: Q91XA2 Mouse
- Unigene: 494337 Human
- Unigene: 171335 Mouse
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Alternative names
- bA379P1.3 antibody
- C9orf155 antibody
- Chromosome 9 open reading frame 155 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GOLPH2 antibody (ab92612)Immunofluorescence of GOLPH2 in rat brain tissue with ab92612 at 20 ug/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody (ab92612)Immunohistochemistry of rat brain tissue with ab92612 at 2.5 μg/mL.
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Western blot - Anti-GOLPH2 antibody (ab92612)Lane 1 : Anti-GOLPH2 antibody (ab92612) at 0.25 µg/ml
Lane 2 : Anti-GOLPH2 antibody (ab92612) at 0.5 µg/ml
All lanes : Rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 45 kDa
Protocols
References
ab92612 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"