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    golph2-antibody-ab92612.pdf

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Signal Transduction Protein Trafficking Organelle Proteins
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Anti-GOLPH2 antibody (ab92612)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody (ab92612)
  • Immunocytochemistry/ Immunofluorescence - Anti-GOLPH2 antibody (ab92612)
  • Western blot - Anti-GOLPH2 antibody (ab92612)

Key features and details

  • Rabbit polyclonal to GOLPH2
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Rat
  • Isotype: IgG

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Protein
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Recombinant Human GOLPH2 protein (ab176033)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-GOLPH2 antibody
    See all GOLPH2 primary antibodies
  • Description

    Rabbit polyclonal to GOLPH2
  • Host species

    Rabbit
  • Specificity

    ab92612 will not recognize GOLPH1 or GOLPH3.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Rat
    IHC-P
    Rat
    WB
    Rat
    See all applications and species data
  • Immunogen

    Synthetic 18 amino acid peptide from near the center of Human GOLPH2 (GenBank accession no. EAW62705).

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins
    • Signal Transduction
    • Protein Trafficking
    • Golgi Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human GOLPH2 protein (ab176033)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Rat
IHC-P
Rat
WB
Rat
Application Abreviews Notes
ICC/IF
Use a concentration of 20 µg/ml.
WB
Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 45 kDa.
IHC-P
Use at an assay dependent concentration.
Notes
ICC/IF
Use a concentration of 20 µg/ml.
WB
Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 45 kDa.
IHC-P
Use at an assay dependent concentration.

Target

  • Function

    Unknown. Cellular response protein to viral infection.
  • Tissue specificity

    Widely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), it is strongly expressed in hepatocyte-derived syncitial giant cells. Constitutively expressed by biliary epithelial cells but not by hepatocytes.
  • Sequence similarities

    Belongs to the GOLM1/CASC4 family.
  • Post-translational
    modifications

    Glycosylated.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization

    Golgi apparatus > cis-Golgi network membrane. Early Golgi. Cycles via the cell surface and endosomes upon lumenal pH disruption.
  • Target information above from: UniProt accession Q8NBJ4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 680692 Rat
    • Alternative names

      • bA379P1.3 antibody
      • C9orf155 antibody
      • Chromosome 9 open reading frame 155 antibody
      • Golgi membrane protein 1 antibody
      • Golgi membrane protein GP73 antibody
      • Golgi phosphoprotein 2 antibody
      • Golgi protein 73 kD antibody
      • Golgi protein 73kD antibody
      • GOLM 1 antibody
      • GOLM1 antibody
      • GOLM1_HUMAN antibody
      • GOLPH 2 antibody
      • GOLPH2 antibody
      • GP 73 antibody
      • GP73 antibody
      • PSEC0257 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody (ab92612)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody (ab92612)

      Immunohistochemistry of rat brain tissue with ab92612 at 2.5 μg/mL.

    • Immunocytochemistry/ Immunofluorescence - Anti-GOLPH2 antibody (ab92612)
      Immunocytochemistry/ Immunofluorescence - Anti-GOLPH2 antibody (ab92612)

      Immunofluorescence of GOLPH2 in rat brain tissue with ab92612 at 20 ug/mL.

    • Western blot - Anti-GOLPH2 antibody (ab92612)
      Western blot - Anti-GOLPH2 antibody (ab92612)
      Lane 1 : Anti-GOLPH2 antibody (ab92612) at 0.25 µg/ml
      Lane 2 : Anti-GOLPH2 antibody (ab92612) at 0.5 µg/ml

      All lanes : Rat brain tissue lysate

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 45 kDa

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab92612? Please let us know so that we can cite the reference in this datasheet.

    ab92612 has not yet been referenced specifically in any publications.

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