Anti-GOLPH2 antibody (ab92612)
Key features and details
- Rabbit polyclonal to GOLPH2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-GOLPH2 antibody
See all GOLPH2 primary antibodies -
Description
Rabbit polyclonal to GOLPH2 -
Host species
Rabbit -
Specificity
ab92612 will not recognize GOLPH1 or GOLPH3. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatIHC-P RatWB Rat
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Immunogen
Synthetic 18 amino acid peptide from near the center of Human GOLPH2 (GenBank accession no. EAW62705).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Rat
|
| IHC-P |
Rat
|
| WB |
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
|
|
| WB |
Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 45 kDa.
|
|
| IHC-P |
Use at an assay dependent concentration.
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| Notes |
|---|
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ICC/IF
Use a concentration of 20 µg/ml. |
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WB
Use a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 45 kDa. |
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IHC-P
Use at an assay dependent concentration. |
Target
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Function
Unknown. Cellular response protein to viral infection. -
Tissue specificity
Widely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), it is strongly expressed in hepatocyte-derived syncitial giant cells. Constitutively expressed by biliary epithelial cells but not by hepatocytes. -
Sequence similarities
Belongs to the GOLM1/CASC4 family. -
Post-translational
modificationsGlycosylated.
Phosphorylation sites are present in the extracelllular medium. -
Cellular localization
Golgi apparatus > cis-Golgi network membrane. Early Golgi. Cycles via the cell surface and endosomes upon lumenal pH disruption. - Information by UniProt
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Database links
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Alternative names
- bA379P1.3 antibody
- C9orf155 antibody
- Chromosome 9 open reading frame 155 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH2 antibody (ab92612)
Immunohistochemistry of rat brain tissue with ab92612 at 2.5 μg/mL.
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Immunocytochemistry/ Immunofluorescence - Anti-GOLPH2 antibody (ab92612)Immunofluorescence of GOLPH2 in rat brain tissue with ab92612 at 20 ug/mL.
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Western blot - Anti-GOLPH2 antibody (ab92612)Lane 1 : Anti-GOLPH2 antibody (ab92612) at 0.25 µg/ml
Lane 2 : Anti-GOLPH2 antibody (ab92612) at 0.5 µg/ml
All lanes : Rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 45 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab92612 has not yet been referenced specifically in any publications.