Overview

  • Product name

    Anti-GOLPH2 antibody [EPR3606]
    See all GOLPH2 primary antibodies
  • Description

    Rabbit monoclonal [EPR3606] to GOLPH2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GOLPH2 aa 300-400. The exact sequence is proprietary.

  • Positive control

    • LnCaP and SH-SY5Y cell lysates; Human colon tissue
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1000 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Unknown. Cellular response protein to viral infection.
    • Tissue specificity

      Widely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), it is strongly expressed in hepatocyte-derived syncitial giant cells. Constitutively expressed by biliary epithelial cells but not by hepatocytes.
    • Sequence similarities

      Belongs to the GOLM1/CASC4 family.
    • Post-translational
      modifications

      Glycosylated.
      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization

      Golgi apparatus > cis-Golgi network membrane. Early Golgi. Cycles via the cell surface and endosomes upon lumenal pH disruption.
    • Information by UniProt
    • Database links

    • Alternative names

      • bA379P1.3 antibody
      • C9orf155 antibody
      • Chromosome 9 open reading frame 155 antibody
      • Golgi membrane protein 1 antibody
      • Golgi membrane protein GP73 antibody
      • Golgi phosphoprotein 2 antibody
      • Golgi protein 73 kD antibody
      • Golgi protein 73kD antibody
      • GOLM 1 antibody
      • GOLM1 antibody
      • GOLM1_HUMAN antibody
      • GOLPH 2 antibody
      • GOLPH2 antibody
      • GP 73 antibody
      • GP73 antibody
      • PSEC0257 antibody
      see all

    Images

    • Immunohistochemical analysis of GOLPH2 in paraffin-embedded Human colon tissue using ab109628 at 1/500 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Overlay histogram showing HeLa cells stained with ab109628 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109628, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-GOLPH2 antibody [EPR3606] (ab109628) at 1/1000 dilution

      Lane 1 : LnCaP cell lysate
      Lane 2 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 45 kDa

    • ab109628 staining GOLPH2 in Human small intestine by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

      Tissue was fixed with paraformaldehyde and blocked with 5% milk for 30 min at 37°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 37°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

      See Abreview

    References

    This product has been referenced in:

    • Yang HJ  et al. GP73 promotes invasion and metastasis of bladder cancer by regulating the epithelial-mesenchymal transition through the TGF-ß1/Smad2 signalling pathway. J Cell Mol Med 22:1650-1665 (2018). WB ; Human . Read more (PubMed: 29349903) »
    • Duan J  et al. GOLPH2, a gene downstream of ras signaling, promotes the progression of pancreatic ductal adenocarcinoma. Mol Med Rep 17:4187-4194 (2018). Read more (PubMed: 29344673) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (small intestine)
    Specification
    small intestine
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C

    Abcam user community

    Verified customer

    Submitted Sep 06 2012

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