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    golph3midas-antibody-ab113649.pdf

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Tags & Cell Markers Subcellular Markers Organelles Golgi
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Anti-GOLPH3/MIDAS antibody (ab113649)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH3/MIDAS antibody (ab113649)
  • Western blot - Anti-GOLPH3/MIDAS antibody (ab113649)

Key features and details

  • Rabbit polyclonal to GOLPH3/MIDAS
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-GOLPH3/MIDAS antibody
    See all GOLPH3/MIDAS primary antibodies
  • Description

    Rabbit polyclonal to GOLPH3/MIDAS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human GOLPH3/MIDAS (N terminal) conjugated to keyhole limpet haemocyanin.

  • General notes

     This product was previously labelled as GOLPH3

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Golgi
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Coat Proteins
    • Signal Transduction
    • Protein Trafficking
    • Golgi Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab113649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 34 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene.
  • Tissue specificity

    Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers.
  • Sequence similarities

    Belongs to the GOLPH3/VPS74 family.
  • Post-translational
    modifications

    Phosphorylated.
  • Cellular localization

    Cell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space.
  • Target information above from: UniProt accession Q9H4A6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 64083 Human
    • Entrez Gene: 66629 Mouse
    • Entrez Gene: 78961 Rat
    • Omim: 612207 Human
    • SwissProt: Q9H4A6 Human
    • SwissProt: Q9CRA5 Mouse
    • SwissProt: Q9ERE4 Rat
    • Unigene: 408909 Human
    • Unigene: 250936 Mouse
    • Unigene: 64519 Rat
    see all
  • Alternative names

    • Coat protein antibody
    • Coat protein GPP34 antibody
    • FLJ90675 antibody
    • Golgi associated protein antibody
    • Golgi peripheral membrane protein 1, 34 kDa antibody
    • Golgi phosphoprotein 3 (coat protein) antibody
    • Golgi phosphoprotein 3 antibody
    • Golgi protein antibody
    • GOLP3_HUMAN antibody
    • Golph3 antibody
    • GOPP1 antibody
    • GPP34 antibody
    • MIDAS antibody
    • Mitochondrial DNA absence factor antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH3/MIDAS antibody (ab113649)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH3/MIDAS antibody (ab113649)

    ab113649, at 5 µg/ml, staining GOLPH3/MIDAS in Formalin fixed, Paraffin embedded Human prostate tissue by Immunohistochemistry.

  • Western blot - Anti-GOLPH3/MIDAS antibody (ab113649)
    Western blot - Anti-GOLPH3/MIDAS antibody (ab113649)
    Lane 1 : Anti-GOLPH3/MIDAS antibody (ab113649) at 0.5 µg/ml
    Lane 2 : Anti-GOLPH3/MIDAS antibody (ab113649) at 1 µg/ml

    All lanes : Rat lung tissue lysate

    Predicted band size: 34 kDa

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab113649? Please let us know so that we can cite the reference in this datasheet.

    ab113649 has been referenced in 1 publication.

    • El-Maqsoud NMRA  et al. GOLPH3 and YB-1 Are Novel Markers Correlating With Poor Prognosis in Prostate Cancer. World J Oncol 6:473-484 (2015). PubMed: 28983350

    Customer reviews and Q&As

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