Anti-GOLPH3/MIDAS antibody (ab113649)

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Overview

  • Product name

    Anti-GOLPH3/MIDAS antibody
    See all GOLPH3/MIDAS primary antibodies

  • Description

    Rabbit polyclonal to GOLPH3/MIDAS

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide corresponding to Human GOLPH3/MIDAS (N terminal) conjugated to keyhole limpet haemocyanin.

  • Positive control

    • Human prostate tissue; Rat lung tissue lysate.

  • General notes

     This product was previously labelled as GOLPH3

     

Properties

Applications

Our Abpromise guarantee covers the use of ab113649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 34 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene.

  • Tissue specificity

    Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers.

  • Sequence similarities

    Belongs to the GOLPH3/VPS74 family.

  • Post-translational
    modifications

    Phosphorylated.

  • Cellular localization

    Cell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Coat protein antibody
    • Coat protein GPP34 antibody
    • FLJ90675 antibody
    • Golgi associated protein antibody
    • Golgi peripheral membrane protein 1, 34 kDa antibody
    • Golgi phosphoprotein 3 (coat protein) antibody
    • Golgi phosphoprotein 3 antibody
    • Golgi protein antibody
    • GOLP3_HUMAN antibody
    • Golph3 antibody
    • GOPP1 antibody
    • GPP34 antibody
    • MIDAS antibody
    • Mitochondrial DNA absence factor antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH3/MIDAS antibody (ab113649)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH3/MIDAS antibody (ab113649)

    ab113649, at 5 µg/ml, staining GOLPH3/MIDAS in Formalin fixed, Paraffin embedded Human prostate tissue by Immunohistochemistry.

  • Western blot - Anti-GOLPH3/MIDAS antibody (ab113649)
    Western blot - Anti-GOLPH3/MIDAS antibody (ab113649)
    Lane 1 : Anti-GOLPH3/MIDAS antibody (ab113649) at 0.5 µg/ml
    Lane 2 : Anti-GOLPH3/MIDAS antibody (ab113649) at 1 µg/ml

    All lanes : Rat lung tissue lysate

    Predicted band size: 34 kDa

References

This product has been referenced in:

  • El-Maqsoud NMRA  et al. GOLPH3 and YB-1 Are Novel Markers Correlating With Poor Prognosis in Prostate Cancer. World J Oncol 6:473-484 (2015). Read more (PubMed: 28983350) »
See 1 Publication for this product

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