Key features and details
- Rabbit polyclonal to GOLPH3/MIDAS
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-GOLPH3/MIDAS antibody
See all GOLPH3/MIDAS primary antibodies
DescriptionRabbit polyclonal to GOLPH3/MIDAS
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human GOLPH3/MIDAS (N terminal) conjugated to keyhole limpet haemocyanin.
This product was previously labelled as GOLPH3
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab113649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 34 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionInvolved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene.
Tissue specificityDetected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers.
Sequence similaritiesBelongs to the GOLPH3/VPS74 family.
Cellular localizationCell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space.
- Information by UniProt
- Coat protein antibody
- Coat protein GPP34 antibody
- FLJ90675 antibody
ab113649, at 5 µg/ml, staining GOLPH3/MIDAS in Formalin fixed, Paraffin embedded Human prostate tissue by Immunohistochemistry.
Lane 1 : Anti-GOLPH3/MIDAS antibody (ab113649) at 0.5 µg/ml
Lane 2 : Anti-GOLPH3/MIDAS antibody (ab113649) at 1 µg/ml
All lanes : Rat lung tissue lysate
Predicted band size: 34 kDa
ab113649 has been referenced in 1 publication.
- El-Maqsoud NMRA et al. GOLPH3 and YB-1 Are Novel Markers Correlating With Poor Prognosis in Prostate Cancer. World J Oncol 6:473-484 (2015). PubMed: 28983350