Anti-GOLPH3/MIDAS antibody (ab113649)
Key features and details
- Rabbit polyclonal to GOLPH3/MIDAS
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-GOLPH3/MIDAS antibody
See all GOLPH3/MIDAS primary antibodies -
Description
Rabbit polyclonal to GOLPH3/MIDAS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human GOLPH3/MIDAS (N terminal) conjugated to keyhole limpet haemocyanin.
-
General notes
This product was previously labelled as GOLPH3
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab113649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 34 kDa. | |
IHC-P | Use a concentration of 5 µg/ml. |
Target
-
Function
Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene. -
Tissue specificity
Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers. -
Sequence similarities
Belongs to the GOLPH3/VPS74 family. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Cell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space. - Information by UniProt
-
Database links
- Entrez Gene: 64083 Human
- Entrez Gene: 66629 Mouse
- Entrez Gene: 78961 Rat
- Omim: 612207 Human
- SwissProt: Q9H4A6 Human
- SwissProt: Q9CRA5 Mouse
- SwissProt: Q9ERE4 Rat
- Unigene: 408909 Human
see all -
Alternative names
- Coat protein antibody
- Coat protein GPP34 antibody
- FLJ90675 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH3/MIDAS antibody (ab113649)
ab113649, at 5 µg/ml, staining GOLPH3/MIDAS in Formalin fixed, Paraffin embedded Human prostate tissue by Immunohistochemistry.
-
Lane 1 : Anti-GOLPH3/MIDAS antibody (ab113649) at 0.5 µg/ml
Lane 2 : Anti-GOLPH3/MIDAS antibody (ab113649) at 1 µg/ml
All lanes : Rat lung tissue lysate
Predicted band size: 34 kDa
Protocols
Datasheets and documents
References (1)
ab113649 has been referenced in 1 publication.
- El-Maqsoud NMRA et al. GOLPH3 and YB-1 Are Novel Markers Correlating With Poor Prognosis in Prostate Cancer. World J Oncol 6:473-484 (2015). PubMed: 28983350