Key features and details
- Rabbit polyclonal to GOLPH3/MIDAS
- Suitable for: IHC-P, WB, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GOLPH3/MIDAS antibody
See all GOLPH3/MIDAS primary antibodies
DescriptionRabbit polyclonal to GOLPH3/MIDAS
Tested applicationsSuitable for: IHC-P, WB, ICC/IF, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- WB: HeLa, HEK-293 and A549 whole cell lysates. IHC-P: Human prostate cancer and prosate tissues. ICC/IF: HepG2 cells. IP: HeLa whole cell lysate.
This product was previously labelled as GOLPH3
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Predicted molecular weight: 34 kDa.|
|ICC/IF||1/20 - 1/200.|
|IP||1/200 - 1/2000.|
FunctionInvolved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene.
Tissue specificityDetected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers.
Sequence similaritiesBelongs to the GOLPH3/VPS74 family.
Cellular localizationCell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space.
- Information by UniProt
- Coat protein antibody
- Coat protein GPP34 antibody
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Paraffin-embedded human prostate cancer tissue stained for GOLPH3/MIDAS using ab236296 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-GOLPH3/MIDAS antibody (ab236296) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34 kDa
Paraffin-embedded human prostate tissue stained for GOLPH3/MIDAS using ab236296 at 1/100 dilution in immunohistochemical analysis.
4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for GOLPH3/MIDAS(green) using ab236296 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.
GOLPH3/MIDAS was immunoprecipitated from 500 µg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab236296 at 1/200 dilution.
Lane 1: Rabbit control IgG IP in HeLa whole cell lysate.
Lane 2: ab236296 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 10 µg (Input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236296 has not yet been referenced specifically in any publications.