Key features and details
- Rabbit polyclonal to GOLPH3/MIDAS
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GOLPH3/MIDAS antibody
See all GOLPH3/MIDAS primary antibodies
DescriptionRabbit polyclonal to GOLPH3/MIDAS
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human GOLPH3/MIDAS aa 25-75. The exact sequence is proprietary.
Database link: Q9H4A6
- IP: HeLa whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264407 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
Use 15-20 µl/mg lysate.
FunctionInvolved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene.
Tissue specificityDetected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers.
Sequence similaritiesBelongs to the GOLPH3/VPS74 family.
Cellular localizationCell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space.
- Information by UniProt
- Coat protein antibody
- Coat protein GPP34 antibody
- FLJ90675 antibody
GOLPH3/MIDAS was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264407 at 15 µl per reaction. Western blot was performed from the immunoprecipitate using an alternative Anti-GOLPH3/MIDAS antibody.
Lane 1: ab264407 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 seconds.
Lysates prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264407 has not yet been referenced specifically in any publications.