Product nameAnti-GOLPH3/MIDAS antibody
See all GOLPH3/MIDAS primary antibodies
DescriptionMouse polyclonal to GOLPH3/MIDAS
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Full length protein corresponding to Human GOLPH3/MIDAS.
Database link: NP_071413
- GOLPH3/MIDAS transfected 293T cell lysate
This product was previously labelled as GOLPH3
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab69171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa).|
FunctionInvolved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene.
Tissue specificityDetected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers.
Sequence similaritiesBelongs to the GOLPH3/VPS74 family.
Cellular localizationCell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space.
- Information by UniProt
- Coat protein antibody
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All lanes : Anti-GOLPH3/MIDAS antibody (ab69171) at 1/500 dilution
Lane 1 : GOLPH3/MIDAS transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 34 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
ab69171 staining GOLPH3/MIDAS in human KG1a cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in formaldehyde, blocked with 2% serum for 1 hour at 22°C and then incubated with ab69171 at a 1/100 dilution for 1 hour at 22°C. The secondary used was a DyLight 488 conjugated donkey anti-mouse polyclonal used at a 1/200 dilution.Blue is nucleus staining by DAPI, green is GOLPH3/MIDAS.
This product has been referenced in:
- Li H et al. GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancer. J Transl Med 10:168 (2012). WB . Read more (PubMed: 22905766) »
- Wang JH et al. High expression of GOLPH3 in esophageal squamous cell carcinoma correlates with poor prognosis. PLoS One 7:e45622 (2012). WB, IHC . Read more (PubMed: 23056210) »