Overview

  • Product name

    Anti-GOLPH3/MIDAS antibody
    See all GOLPH3/MIDAS primary antibodies
  • Description

    Rabbit polyclonal to GOLPH3/MIDAS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide corresponding to Human GOLPH3/MIDAS (N terminal).
    Database link: AAH12123

  • Positive control

    • Rat Lung Tissue Lysate
  • General notes

     This product was previously labelled as GOLPH3

     

Properties

Applications

Our Abpromise guarantee covers the use of ab91492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/150.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 34 kDa.
ELISA Use at an assay dependent dilution.

Target

  • Function

    Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene.
  • Tissue specificity

    Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers.
  • Sequence similarities

    Belongs to the GOLPH3/VPS74 family.
  • Post-translational
    modifications

    Phosphorylated.
  • Cellular localization

    Cell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space.
  • Information by UniProt
  • Database links

  • Alternative names

    • Coat protein antibody
    • Coat protein GPP34 antibody
    • FLJ90675 antibody
    • Golgi associated protein antibody
    • Golgi peripheral membrane protein 1, 34 kDa antibody
    • Golgi phosphoprotein 3 (coat protein) antibody
    • Golgi phosphoprotein 3 antibody
    • Golgi protein antibody
    • GOLP3_HUMAN antibody
    • Golph3 antibody
    • GOPP1 antibody
    • GPP34 antibody
    • MIDAS antibody
    • Mitochondrial DNA absence factor antibody
    see all

Images

  • Lane 1 : Anti-GOLPH3/MIDAS antibody (ab91492) at 0.5 µg/ml
    Lane 2 : Anti-GOLPH3/MIDAS antibody (ab91492) at 1 µg/ml

    All lanes : rat lung tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 34 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 38 kDa, 42 kDa, 50 kDa. We are unsure as to the identity of these extra bands.

  • ab91492 staining GOLPH3/MIDAS in human K562 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in formaldehyde, blocked with 2% serum for 1 hour at 22°C and then incubated with ab91492 at a 1/150 dilution for 1 hour at 22°C. The secondary used was a DyLight 594 conjugated donkey anti-rabbit polyclonal used at a 1/200 dilution.Blue is nucleus staining by DAPI, red is GOLPH3/MIDAS.

    See Abreview

  • ab91492, staining GOLPH3/MIDAS (red) in HCV-infected Huh 7.5.1 cells, by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed in 3% paraformaldehyde, permeabilized and blocked in antibody-binding buffer for 2 hours at 4°C. Samples were incubated with primary antibody and a fluorescence-conjugated donkey anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

  • ab91492, staining GOLPH3/MIDAS in bovine aortic endothelial cells, by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100/PBS for 15 min at RT and blocked with 5% donkey serum/0.3% BSA/PBS for 30 min at RT. Samples were incubated with primary antibody at 1/100 dilution overnight at 4°C. An AlexaFlour®568-conjugated anti-rabbit IgG (1/500) was used as the secondary antibody.

  • Immunofluorescence of GOLPH3/MIDAS in Rat Lung cells using ab91492 at 20 ug/ml.

References

This product has been referenced in:

  • Peñalver-González B  et al. Golgi Oncoprotein GOLPH3 Gene Expression Is Regulated by Functional E2F and CREB/ATF Promoter Elements. Genes (Basel) 10:N/A (2019). Read more (PubMed: 30934642) »
  • Song Q  et al. ATF-3/miR-590/GOLPH3 signaling pathway regulates proliferation of breast cancer. BMC Cancer 18:255 (2018). Read more (PubMed: 29534690) »
See all 6 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (K562)
Permeabilization
No
Specification
K562
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Mar 14 2012

Question
Answer

DISCOUNT CODE: ***
Expiration date: July 13th, 2012

I am very pleased to hear you would like to accept our offer and test ab91492 in ICC/IF. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for ICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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