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    golph3midas-antibody-ab91492.pdf

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Tags & Cell Markers Subcellular Markers Organelles Golgi
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Anti-GOLPH3/MIDAS antibody (ab91492)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (7)

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Western blot - Anti-GOLPH3/MIDAS antibody (ab91492)
  • Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (ab91492)
  • Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (ab91492)
  • Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (ab91492)
  • Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (ab91492)

Key features and details

  • Rabbit polyclonal to GOLPH3/MIDAS
  • Suitable for: ICC, WB
  • Reacts with: Rat
  • Isotype: IgG

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Overview

  • Product name

    Anti-GOLPH3/MIDAS antibody
    See all GOLPH3/MIDAS primary antibodies
  • Description

    Rabbit polyclonal to GOLPH3/MIDAS
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC
    Rat
    WB
    Rat
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human GOLPH3/MIDAS (N terminal).
    Database link: AAH12123

  • General notes

     This product was previously labelled as GOLPH3

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Golgi
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Coat Proteins
    • Signal Transduction
    • Protein Trafficking
    • Golgi Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab91492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC
Rat
WB
Rat
Application Abreviews Notes
ICC
Use at an assay dependent concentration.
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 34 kDa.
Notes
ICC
Use at an assay dependent concentration.
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 34 kDa.

Target

  • Function

    Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene.
  • Tissue specificity

    Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers.
  • Sequence similarities

    Belongs to the GOLPH3/VPS74 family.
  • Post-translational
    modifications

    Phosphorylated.
  • Cellular localization

    Cell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space.
  • Target information above from: UniProt accession Q9H4A6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 78961 Rat
    • SwissProt: Q9ERE4 Rat
    • Unigene: 64519 Rat
    • Alternative names

      • Coat protein antibody
      • Coat protein GPP34 antibody
      • FLJ90675 antibody
      • Golgi associated protein antibody
      • Golgi peripheral membrane protein 1, 34 kDa antibody
      • Golgi phosphoprotein 3 (coat protein) antibody
      • Golgi phosphoprotein 3 antibody
      • Golgi protein antibody
      • GOLP3_HUMAN antibody
      • Golph3 antibody
      • GOPP1 antibody
      • GPP34 antibody
      • MIDAS antibody
      • Mitochondrial DNA absence factor antibody
      see all

    Images

    • Western blot - Anti-GOLPH3/MIDAS antibody (ab91492)
      Western blot - Anti-GOLPH3/MIDAS antibody (ab91492)
      Lane 1 : Anti-GOLPH3/MIDAS antibody (ab91492) at 0.5 µg/ml
      Lane 2 : Anti-GOLPH3/MIDAS antibody (ab91492) at 1 µg/ml

      All lanes : rat lung tissue lysate

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 34 kDa
      Observed band size: 28 kDa why is the actual band size different from the predicted?
      Additional bands at: 38 kDa, 42 kDa, 50 kDa. We are unsure as to the identity of these extra bands.

    • Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (ab91492)
      Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (ab91492)Image courtesy of Armen Petrosyan by Abreview.

      ab91492 staining GOLPH3/MIDAS in human K562 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in formaldehyde, blocked with 2% serum for 1 hour at 22°C and then incubated with ab91492 at a 1/150 dilution for 1 hour at 22°C. The secondary used was a DyLight 594 conjugated donkey anti-rabbit polyclonal used at a 1/200 dilution.Blue is nucleus staining by DAPI, red is GOLPH3/MIDAS.

      See Abreview

    • Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (ab91492)
      Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (ab91492)Image from Bishé B et al., J Biol Chem 287:27637-47 (2012)Fig 6.; doi: 10.1074/jbc.M112.346569; August 10, 2012, The Journal of Biological Chemistry, 287, 27637-27647.

      ab91492, staining GOLPH3/MIDAS (red) in HCV-infected Huh 7.5.1 cells, by Immunocytochemistry/ Immunofluorescence.

      Cells were fixed in 3% paraformaldehyde, permeabilized and blocked in antibody-binding buffer for 2 hours at 4°C. Samples were incubated with primary antibody and a fluorescence-conjugated donkey anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

    • Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (ab91492)
      Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (ab91492)Image from Sangwung P et al., PLoS One. 2012;7(2):e31564. Epub 2012 Feb 21. Fig 3.; doi:10.1371/journal.pone.0031564; February 21, 2012, PLoS ONE 7(2): e31564.

      ab91492, staining GOLPH3/MIDAS in bovine aortic endothelial cells, by Immunocytochemistry/ Immunofluorescence.

      Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100/PBS for 15 min at RT and blocked with 5% donkey serum/0.3% BSA/PBS for 30 min at RT. Samples were incubated with primary antibody at 1/100 dilution overnight at 4°C. An AlexaFlour®568-conjugated anti-rabbit IgG (1/500) was used as the secondary antibody.

    • Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (ab91492)
      Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (ab91492)

      Immunofluorescence of GOLPH3/MIDAS in Rat Lung cells using ab91492 at 20 ug/ml.

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (7)

    Publishing research using ab91492? Please let us know so that we can cite the reference in this datasheet.

    ab91492 has been referenced in 7 publications.

    • Zhao C  et al. GOLPH3 Promotes Angiogenesis of Lung Adenocarcinoma by Regulating the Wnt/ß-Catenin Signaling Pathway. Onco Targets Ther 13:6265-6277 (2020). PubMed: 32636646
    • Peñalver-González B  et al. Golgi Oncoprotein GOLPH3 Gene Expression Is Regulated by Functional E2F and CREB/ATF Promoter Elements. Genes (Basel) 10:N/A (2019). PubMed: 30934642
    • Song Q  et al. ATF-3/miR-590/GOLPH3 signaling pathway regulates proliferation of breast cancer. BMC Cancer 18:255 (2018). PubMed: 29534690
    • Tang S  et al. Expression of GOLPH3 in patients with non-small cell lung cancer and xenografts models. Oncol Lett 15:7555-7562 (2018). PubMed: 29849795
    • Tang S  et al. GOLPH3: a novel biomarker that correlates with poor survival and resistance to chemotherapy in breast cancer. Oncotarget 8:105155-105169 (2017). PubMed: 29285241
    • Sangwung P  et al. Proteomic identification of S-nitrosylated Golgi proteins: new insights into endothelial cell regulation by eNOS-derived NO. PLoS One 7:e31564 (2012). ICC/IF ; Cow . PubMed: 22363674
    • Bishé B  et al. Role of Phosphatidylinositol 4-Phosphate (PI4P) and Its Binding Protein GOLPH3 in Hepatitis C Virus Secretion. J Biol Chem 287:27637-47 (2012). ICC/IF ; Human . PubMed: 22745132

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-GOLPH3/MIDAS antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (K562)
    Permeabilization
    No
    Specification
    K562
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Dr. Armen Petrosyan

    Verified customer

    Submitted Mar 14 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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