Anti-GOLPH3/MIDAS antibody (ab91492)
Key features and details
- Rabbit polyclonal to GOLPH3/MIDAS
- Suitable for: ICC, WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-GOLPH3/MIDAS antibody
See all GOLPH3/MIDAS primary antibodies -
Description
Rabbit polyclonal to GOLPH3/MIDAS -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC RatWB Rat
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Immunogen
Synthetic peptide corresponding to Human GOLPH3/MIDAS (N terminal).
Database link: AAH12123 -
General notes
This product was previously labelled as GOLPH3
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC |
Rat
|
| WB |
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
|
|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 34 kDa.
|
| Notes |
|---|
|
ICC
Use at an assay dependent concentration. |
|
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 34 kDa. |
Target
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Function
Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene. -
Tissue specificity
Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers. -
Sequence similarities
Belongs to the GOLPH3/VPS74 family. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Cell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space. - Information by UniProt
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Database links
- Entrez Gene: 78961 Rat
- SwissProt: Q9ERE4 Rat
- Unigene: 64519 Rat
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Alternative names
- Coat protein antibody
- Coat protein GPP34 antibody
- FLJ90675 antibody
see all
Images
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Western blot - Anti-GOLPH3/MIDAS antibody (ab91492)Lane 1 : Anti-GOLPH3/MIDAS antibody (ab91492) at 0.5 µg/ml
Lane 2 : Anti-GOLPH3/MIDAS antibody (ab91492) at 1 µg/ml
All lanes : rat lung tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 34 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa, 42 kDa, 50 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (ab91492)Image courtesy of Armen Petrosyan by Abreview.ab91492 staining GOLPH3/MIDAS in human K562 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in formaldehyde, blocked with 2% serum for 1 hour at 22°C and then incubated with ab91492 at a 1/150 dilution for 1 hour at 22°C. The secondary used was a DyLight 594 conjugated donkey anti-rabbit polyclonal used at a 1/200 dilution.Blue is nucleus staining by DAPI, red is GOLPH3/MIDAS.
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Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (ab91492)Image from Bishé B et al., J Biol Chem 287:27637-47 (2012)Fig 6.; doi: 10.1074/jbc.M112.346569; August 10, 2012, The Journal of Biological Chemistry, 287, 27637-27647.ab91492, staining GOLPH3/MIDAS (red) in HCV-infected Huh 7.5.1 cells, by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in 3% paraformaldehyde, permeabilized and blocked in antibody-binding buffer for 2 hours at 4°C. Samples were incubated with primary antibody and a fluorescence-conjugated donkey anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI (blue). -
Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (ab91492)Image from Sangwung P et al., PLoS One. 2012;7(2):e31564. Epub 2012 Feb 21. Fig 3.; doi:10.1371/journal.pone.0031564; February 21, 2012, PLoS ONE 7(2): e31564.ab91492, staining GOLPH3/MIDAS in bovine aortic endothelial cells, by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100/PBS for 15 min at RT and blocked with 5% donkey serum/0.3% BSA/PBS for 30 min at RT. Samples were incubated with primary antibody at 1/100 dilution overnight at 4°C. An AlexaFlour®568-conjugated anti-rabbit IgG (1/500) was used as the secondary antibody. -
Immunocytochemistry - Anti-GOLPH3/MIDAS antibody (ab91492)Immunofluorescence of GOLPH3/MIDAS in Rat Lung cells using ab91492 at 20 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab91492 has been referenced in 7 publications.
- Zhao C et al. GOLPH3 Promotes Angiogenesis of Lung Adenocarcinoma by Regulating the Wnt/ß-Catenin Signaling Pathway. Onco Targets Ther 13:6265-6277 (2020). PubMed: 32636646
- Peñalver-González B et al. Golgi Oncoprotein GOLPH3 Gene Expression Is Regulated by Functional E2F and CREB/ATF Promoter Elements. Genes (Basel) 10:N/A (2019). PubMed: 30934642
- Song Q et al. ATF-3/miR-590/GOLPH3 signaling pathway regulates proliferation of breast cancer. BMC Cancer 18:255 (2018). PubMed: 29534690
- Tang S et al. Expression of GOLPH3 in patients with non-small cell lung cancer and xenografts models. Oncol Lett 15:7555-7562 (2018). PubMed: 29849795
- Tang S et al. GOLPH3: a novel biomarker that correlates with poor survival and resistance to chemotherapy in breast cancer. Oncotarget 8:105155-105169 (2017). PubMed: 29285241
- Sangwung P et al. Proteomic identification of S-nitrosylated Golgi proteins: new insights into endothelial cell regulation by eNOS-derived NO. PLoS One 7:e31564 (2012). ICC/IF ; Cow . PubMed: 22363674
- Bishé B et al. Role of Phosphatidylinositol 4-Phosphate (PI4P) and Its Binding Protein GOLPH3 in Hepatitis C Virus Secretion. J Biol Chem 287:27637-47 (2012). ICC/IF ; Human . PubMed: 22745132