Anti-GOLPH3/MIDAS antibody (ab91492)
- Datasheet
- References (6)
- Protocols
Overview
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Product name
Anti-GOLPH3/MIDAS antibody
See all GOLPH3/MIDAS primary antibodies -
Description
Rabbit polyclonal to GOLPH3/MIDAS -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, ELISAmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Immunogen
Synthetic peptide corresponding to Human GOLPH3/MIDAS (N terminal).
Database link: AAH12123 -
Positive control
- Rat Lung Tissue Lysate
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General notes
This product was previously labelled as GOLPH3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab91492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/150. | |
| WB | Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 34 kDa. | |
| ELISA | Use at an assay dependent dilution. |
Target
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Function
Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene. -
Tissue specificity
Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers. -
Sequence similarities
Belongs to the GOLPH3/VPS74 family. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Cell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space. - Information by UniProt
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Database links
- Entrez Gene: 531359 Cow
- Entrez Gene: 64083 Human
- Entrez Gene: 66629 Mouse
- Entrez Gene: 78961 Rat
- Omim: 612207 Human
- SwissProt: Q9H4A6 Human
- SwissProt: Q9CRA5 Mouse
- SwissProt: Q9ERE4 Rat
see all -
Alternative names
- Coat protein antibody
- Coat protein GPP34 antibody
- FLJ90675 antibody
see all
Images
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Western blot - Anti-GOLPH3/MIDAS antibody (ab91492)Lane 1 : Anti-GOLPH3/MIDAS antibody (ab91492) at 0.5 µg/ml
Lane 2 : Anti-GOLPH3/MIDAS antibody (ab91492) at 1 µg/ml
All lanes : rat lung tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 34 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa, 42 kDa, 50 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-GOLPH3/MIDAS antibody (ab91492)Image courtesy of Armen Petrosyan by Abreview.ab91492 staining GOLPH3/MIDAS in human K562 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in formaldehyde, blocked with 2% serum for 1 hour at 22°C and then incubated with ab91492 at a 1/150 dilution for 1 hour at 22°C. The secondary used was a DyLight 594 conjugated donkey anti-rabbit polyclonal used at a 1/200 dilution.Blue is nucleus staining by DAPI, red is GOLPH3/MIDAS.
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Immunocytochemistry/ Immunofluorescence - Anti-GOLPH3/MIDAS antibody (ab91492)Image from Bishé B et al., J Biol Chem 287:27637-47 (2012)Fig 6.; doi: 10.1074/jbc.M112.346569; August 10, 2012, The Journal of Biological Chemistry, 287, 27637-27647.ab91492, staining GOLPH3/MIDAS (red) in HCV-infected Huh 7.5.1 cells, by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in 3% paraformaldehyde, permeabilized and blocked in antibody-binding buffer for 2 hours at 4°C. Samples were incubated with primary antibody and a fluorescence-conjugated donkey anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI (blue). -
Immunocytochemistry/ Immunofluorescence - Anti-GOLPH3/MIDAS antibody (ab91492)Image from Sangwung P et al., PLoS One. 2012;7(2):e31564. Epub 2012 Feb 21. Fig 3.; doi:10.1371/journal.pone.0031564; February 21, 2012, PLoS ONE 7(2): e31564.ab91492, staining GOLPH3/MIDAS in bovine aortic endothelial cells, by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100/PBS for 15 min at RT and blocked with 5% donkey serum/0.3% BSA/PBS for 30 min at RT. Samples were incubated with primary antibody at 1/100 dilution overnight at 4°C. An AlexaFlour®568-conjugated anti-rabbit IgG (1/500) was used as the secondary antibody. -
Immunocytochemistry/ Immunofluorescence - Anti-GOLPH3/MIDAS antibody (ab91492)Immunofluorescence of GOLPH3/MIDAS in Rat Lung cells using ab91492 at 20 ug/ml.
Datasheets and documents
References
This product has been referenced in:
- Peñalver-González B et al. Golgi Oncoprotein GOLPH3 Gene Expression Is Regulated by Functional E2F and CREB/ATF Promoter Elements. Genes (Basel) 10:N/A (2019). Read more (PubMed: 30934642) »
- Song Q et al. ATF-3/miR-590/GOLPH3 signaling pathway regulates proliferation of breast cancer. BMC Cancer 18:255 (2018). Read more (PubMed: 29534690) »
