Western blot abreview for Anti-GOLPH3/MIDAS antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Gel Running Conditions
Reduced Denaturing (4-20% tris-glycine)
Loading amount
30 cells
Specification
U2OS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% milk in PBST
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Rabbit IgG, HRP-linked whole Ab
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Negative control
GOLPH3+3L CRISPR KO U2OS
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 38 kDa Non-specific: 30, 45, 100 kDa
Positive control
WT U2OS

Additional data

Additional Notes
Lanes: 1 - ladder, 2 - WT, 3 GOLPH3+3L KO Nitrocellulose membrane Lysis buffer 0.5% triton, 50 mM Tris-HCl pH 7.4, 150 NaCl, 1 mM EDTA, 1 mM PMSF, 1x COMP protease inhibitors.

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Submitted Jun 17 2019

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