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Anti-GOLPH3/MIDAS antibody - C-terminal (ab223772)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH3/MIDAS antibody - C-terminal (ab223772)

    Key features and details

    • Rabbit polyclonal to GOLPH3/MIDAS - C-terminal
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

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    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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    Overview

    • Product name

      Anti-GOLPH3/MIDAS antibody - C-terminal
      See all GOLPH3/MIDAS primary antibodies
    • Description

      Rabbit polyclonal to GOLPH3/MIDAS - C-terminal
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      IHC-P
      Human
      See all applications and species data
    • Immunogen

      Recombinant fragment corresponding to Human GOLPH3/MIDAS aa 200 to the C-terminus.
      Database link: Q9H4A6

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • IHC-P: Human stomach tissue.
    • General notes

       This product was previously labelled as GOLPH3

       

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.20
      Preservative: 0.02% Sodium azide
      Constituents: 40% Glycerol (glycerin, glycerine), PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Tags & Cell Markers
      • Subcellular Markers
      • Organelles
      • Golgi
      • Signal Transduction
      • Protein Trafficking
      • Vesicle Transport
      • Coat Proteins
      • Signal Transduction
      • Protein Trafficking
      • Golgi Proteins

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab223772 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-P
    Human
    All applications
    Mouse
    Rat
    Application Abreviews Notes
    IHC-P
    1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    Notes
    IHC-P
    1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Target

    • Function

      Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene.
    • Tissue specificity

      Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers.
    • Sequence similarities

      Belongs to the GOLPH3/VPS74 family.
    • Post-translational
      modifications

      Phosphorylated.
    • Cellular localization

      Cell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space.
    • Target information above from: UniProt accession Q9H4A6 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 64083 Human
      • Entrez Gene: 66629 Mouse
      • Entrez Gene: 78961 Rat
      • Omim: 612207 Human
      • SwissProt: Q9H4A6 Human
      • SwissProt: Q9CRA5 Mouse
      • SwissProt: Q9ERE4 Rat
      • Unigene: 408909 Human
      • Unigene: 250936 Mouse
      • Unigene: 64519 Rat
      see all
    • Alternative names

      • Coat protein antibody
      • Coat protein GPP34 antibody
      • FLJ90675 antibody
      • Golgi associated protein antibody
      • Golgi peripheral membrane protein 1, 34 kDa antibody
      • Golgi phosphoprotein 3 (coat protein) antibody
      • Golgi phosphoprotein 3 antibody
      • Golgi protein antibody
      • GOLP3_HUMAN antibody
      • Golph3 antibody
      • GOPP1 antibody
      • GPP34 antibody
      • MIDAS antibody
      • Mitochondrial DNA absence factor antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH3/MIDAS antibody - C-terminal (ab223772)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH3/MIDAS antibody - C-terminal (ab223772)

      Paraffin-embedded human stomach tissue stained for GOLPH3/MIDAS using ab223772 at 1/500 dilution in immunohistochemical analysis.

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab223772? Please let us know so that we can cite the reference in this datasheet.

    ab223772 has not yet been referenced specifically in any publications.

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