Product nameAnti-GOLPH4 antibody
See all GOLPH4 primary antibodies
DescriptionRabbit polyclonal to GOLPH4
Tested applicationsSuitable for: IHC-Fr, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Human, Monkey
Recombinant GOLPH4 fusion protein
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferConstituent: Whole serum
Our Abpromise guarantee covers the use of ab28049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Predicted molecular weight: 130 kDa.|
FunctionPlays a role in endosome to Golgi protein trafficking; mediates protein transport along the late endosome-bypass pathway from the early endosome to the Golgi.
Sequence similaritiesBelongs to the GOLIM4 family.
modificationsPhosphorylated probably by c-AMP-dependent kinases in its lumenal part.
O-glycosylated; modified by sialic acid residues.
N-glycosylated; N-glycans are probably of the complex type and modified by sialic acid residues.
Cellular localizationGolgi apparatus > Golgi stack membrane. Endosome membrane. Localizes to cis and medial Golgi cisternae. Probably cycles between early Golgi and distal compartments like endosome.
- Information by UniProt
- 130 kDa golgi localized phosphoprotein antibody
- cis antibody
- cis Golgi localized calcium binding protein antibody
ab28049 staining mouse cortical oligodendrocytes from vibratome sections by IHC-Fr. Sections were fixed in Somogyi (PFA + glutaraldehyde + picric acid) or PLP (Periodate-lysine-PFA) and permeabilized in TX-100, prior to blocking with 2.5% serum for 2 hours at 25°C. The primary antibody was diluted 1/1000 and incubated with the sample for 18 hours. A TRITC conjugated goat anti-rabbit IgG antibody was used as the secondary.
This product has been referenced in:
- Hikita T et al. PAR-3 controls endothelial planar polarity and vascular inflammation under laminar flow. EMBO Rep 19:N/A (2018). Read more (PubMed: 30018153) »
- Liu L et al. Engineering of GlcNAc-1-Phosphotransferase for Production of Highly Phosphorylated Lysosomal Enzymes for Enzyme Replacement Therapy. Mol Ther Methods Clin Dev 5:59-65 (2017). Read more (PubMed: 28480305) »