Overview

  • Product name
  • Description
    Rabbit polyclonal to GOLPH4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human, Monkey
  • Immunogen

    Recombinant GOLPH4 fusion protein

Properties

Applications

Our Abpromise guarantee covers the use of ab28049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/1000.
WB 1/500. Predicted molecular weight: 130 kDa.
ICC/IF 1/400.

Target

  • Function
    Plays a role in endosome to Golgi protein trafficking; mediates protein transport along the late endosome-bypass pathway from the early endosome to the Golgi.
  • Sequence similarities
    Belongs to the GOLIM4 family.
  • Post-translational
    modifications
    Phosphorylated probably by c-AMP-dependent kinases in its lumenal part.
    O-glycosylated; modified by sialic acid residues.
    N-glycosylated; N-glycans are probably of the complex type and modified by sialic acid residues.
  • Cellular localization
    Golgi apparatus > Golgi stack membrane. Endosome membrane. Localizes to cis and medial Golgi cisternae. Probably cycles between early Golgi and distal compartments like endosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • 130 kDa golgi localized phosphoprotein antibody
    • cis antibody
    • cis Golgi localized calcium binding protein antibody
    • GIMPc antibody
    • Golgi integral membrane protein 4 antibody
    • Golgi integral membrane protein antibody
    • Golgi phosphoprotein 4 antibody
    • Golgi phosphoprotein of 130 kDa antibody
    • Golgi-localized phosphoprotein of 130 kDa antibody
    • GOLI4_HUMAN antibody
    • golim4 antibody
    • GOLPH 4 antibody
    • GPP 130 antibody
    • GPP130 antibody
    • P 138 antibody
    • P138 antibody
    • type II Golgi membrane protein antibody
    see all

Images

  • ab28049 staining mouse cortical oligodendrocytes from vibratome sections by IHC-Fr.  Sections were fixed in Somogyi (PFA + glutaraldehyde + picric acid) or PLP (Periodate-lysine-PFA) and permeabilized in TX-100, prior to blocking with 2.5% serum for 2 hours at 25°C.  The primary antibody was diluted 1/1000 and incubated with the sample for 18 hours.  A TRITC conjugated goat anti-rabbit IgG antibody was used as the secondary.

    See Abreview

References

This product has been referenced in:
See all 13 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Sample
Human Cell (ovarian cancer A2780 cell line)
Specification
ovarian cancer A2780 cell line
Permeabilization
Yes - 0.1% triton-X , 5min, RT
Fixative
Paraformaldehyde

Dr. Arpita Kulshrestha

Verified customer

Submitted Aug 25 2014

Answer

Merci de nous avoir contactés et de nous avoir informés du problème avec ab28049. Comme convenu, j'ai mis en place l'envoi d'une unité d'anti-GOLPH4 ab57271. Le numéro de commande de remplacement gratuit de ce produit est ********. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition. J'ai transféré au laboratoire les informations détaillées que vous m'avez communiquées. Je vous contacterai de nouveau dès que j'aurai reçu une réponse. Le protocole Western Blot pour les protéines à haut poids moléculaire est le suivant : Western blots of big proteins such as GOLGB1 can be difficult. - I would suggest to optimize the transfer conditions by increasing the transfer time to 18 or 24 hours (in some examples, for big proteins, the transfer is done for 48 hours) - A transfer at 20-30V for a long period of time is fine but it has been shown that a "boost" at 70-80V for 1 hour at the end of the transfer can be of significant improvement. - You can use slightly more SDS in the transfer buffer, up to 0.05% and reduce the proportion of methanol to 10%. The reason is that methanol washes out the SDS from the protein. J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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Question
Answer

Thank you for contacting us. As discussed over the phone, this antibody is provided as the whole antiserum and the exact concentration is not determined. We would estimate the concentration to be from 1-10 mg/ml.  I hope this information has been of help. If you have any further questions please do not hesitate to ask.

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Intestine)
Specification
Intestine
Fixative
Paraformaldehyde
Antigen retrieval step
Other - Buffer/Enzyme Used: water
Permeabilization
Yes - 15 min in 0.2% PBST
Blocking step
(agent) for 30 minute(s) · Concentration: 20% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Nov 23 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (cortical oligodendrocytes from vibratome sections)
Specification
cortical oligodendrocytes from vibratome sections
Fixative
Somogyi (PFA + glutaraldehyde + picric acid) or PLP (Periodate-lysine-PFA)
Permeabilization
Yes - TX100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C

Dr. Guillermo Estivill-Torrus

Verified customer

Submitted Oct 31 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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