Recombinant Anti-GOLPH4/GPP130 antibody [EPR13439] (ab197595)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-GOLPH4/GPP130 antibody [EPR13439]
See all GOLPH4/GPP130 primary antibodies -
Description
Rabbit monoclonal [EPR13439] to GOLPH4/GPP130 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human GOLPH4/GPP130 aa 50-150. The exact sequence is proprietary.
Database link: O00461 -
Positive control
- WB: HeLa, HepG2 and 293 whole cell lysates. IHC-P: Human renal adenocarcinoma tissue.
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General notes
This product was previously labelled as GOLPH4
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13439 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab197595 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 130 kDa (predicted molecular weight: 82 kDa). | |
| IHC-P | 1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Plays a role in endosome to Golgi protein trafficking; mediates protein transport along the late endosome-bypass pathway from the early endosome to the Golgi. -
Sequence similarities
Belongs to the GOLIM4 family. -
Post-translational
modificationsPhosphorylated probably by c-AMP-dependent kinases in its lumenal part.
O-glycosylated; modified by sialic acid residues.
N-glycosylated; N-glycans are probably of the complex type and modified by sialic acid residues. -
Cellular localization
Golgi apparatus > Golgi stack membrane. Endosome membrane. Localizes to cis and medial Golgi cisternae. Probably cycles between early Golgi and distal compartments like endosome. - Information by UniProt
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Database links
- Entrez Gene: 27333 Human
- Omim: 606805 Human
- SwissProt: O00461 Human
- Unigene: 143600 Human
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Alternative names
- 130 kDa golgi localized phosphoprotein antibody
- cis antibody
- cis Golgi localized calcium binding protein antibody
see all
Images
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![Western blot - Anti-GOLPH4/GPP130 antibody [EPR13439] (ab197595)](https://www.abcam.com/ps/products/197/ab197595/Images/ab197595-237151-anti-golph4-antibody-epr13439-western-blot.jpg)
Western blot - Anti-GOLPH4/GPP130 antibody [EPR13439] (ab197595)Anti-GOLPH4/GPP130 antibody [EPR13439] (ab197595) at 1/2500 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 82 kDa
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature PMID:20130081 and PMID:9201717
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![Western blot - Anti-GOLPH4/GPP130 antibody [EPR13439] (ab197595)](https://www.abcam.com/ps/products/197/ab197595/Images/ab197595-237150-anti-golph4-antibody-epr13439-western-blot.jpg)
Western blot - Anti-GOLPH4/GPP130 antibody [EPR13439] (ab197595)All lanes : Anti-GOLPH4/GPP130 antibody [EPR13439] (ab197595) at 1/5000 dilution
Lane 1 : HepG2(Human liver hepatocellular carcinoma) whole cell lysate
Lane 2 : 293(Human embryonic kidney) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 82 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature (PMID:)20130081 and 9201717
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH4/GPP130 antibody [EPR13439] (ab197595)](https://www.abcam.com/ps/products/197/ab197595/Images/ab197595-237149-anti-golph4-antibody-epr13439-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLPH4/GPP130 antibody [EPR13439] (ab197595)Immunohistochemical analysis of paraffin-embedded Human renal adenocarcinoma tissue labeling GOLPH4/GPP130 with ab197595 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human renal adenocarcinoma tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Datasheets and documents
References
ab197595 has not yet been referenced specifically in any publications.
