Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13445] to GOLPH4/GPP130
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Product nameAnti-GOLPH4/GPP130 antibody [EPR13445]
See all GOLPH4/GPP130 primary antibodies
DescriptionRabbit monoclonal [EPR13445] to GOLPH4/GPP130
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
within Human GOLPH4/GPP130 aa 650 to the C-terminus. The exact sequence is proprietary.
Database link: O00461
- Y79, HeLa and HepG2 cell lysates; HeLa cells.
This product was previously labelled as GOLPH4
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181849 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 130 kDa (predicted molecular weight: 82 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionPlays a role in endosome to Golgi protein trafficking; mediates protein transport along the late endosome-bypass pathway from the early endosome to the Golgi.
Sequence similaritiesBelongs to the GOLIM4 family.
modificationsPhosphorylated probably by c-AMP-dependent kinases in its lumenal part.
O-glycosylated; modified by sialic acid residues.
N-glycosylated; N-glycans are probably of the complex type and modified by sialic acid residues.
Cellular localizationGolgi apparatus > Golgi stack membrane. Endosome membrane. Localizes to cis and medial Golgi cisternae. Probably cycles between early Golgi and distal compartments like endosome.
- Information by UniProt
- 130 kDa golgi localized phosphoprotein antibody
- cis antibody
- cis Golgi localized calcium binding protein antibody
Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling GOLPH4/GPP130 with ab181849 at 1/90 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.
All lanes : Anti-GOLPH4/GPP130 antibody [EPR13445] (ab181849) at 1/20000 dilution
Lane 1 : Y79 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution
Predicted band size: 82 kDa
ab181849 has not yet been referenced specifically in any publications.