Recombinant
RabMAb

Recombinant Anti-GOLPH4/GPP130 antibody [EPR13445] (ab181849)

Overview

  • Product name

    Anti-GOLPH4/GPP130 antibody [EPR13445]
    See all GOLPH4/GPP130 primary antibodies
  • Description

    Rabbit monoclonal [EPR13445] to GOLPH4/GPP130
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    within Human GOLPH4/GPP130 aa 650 to the C-terminus. The exact sequence is proprietary.
    Database link: O00461

  • Positive control

    • Y79, HeLa and HepG2 cell lysates; HeLa cells.
  • General notes

     

     

     This product was previously labelled as GOLPH4

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181849 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 130 kDa (predicted molecular weight: 82 kDa).
Flow Cyt 1/90.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Plays a role in endosome to Golgi protein trafficking; mediates protein transport along the late endosome-bypass pathway from the early endosome to the Golgi.
  • Sequence similarities

    Belongs to the GOLIM4 family.
  • Post-translational
    modifications

    Phosphorylated probably by c-AMP-dependent kinases in its lumenal part.
    O-glycosylated; modified by sialic acid residues.
    N-glycosylated; N-glycans are probably of the complex type and modified by sialic acid residues.
  • Cellular localization

    Golgi apparatus > Golgi stack membrane. Endosome membrane. Localizes to cis and medial Golgi cisternae. Probably cycles between early Golgi and distal compartments like endosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • 130 kDa golgi localized phosphoprotein antibody
    • cis antibody
    • cis Golgi localized calcium binding protein antibody
    • GIMPc antibody
    • Golgi integral membrane protein 4 antibody
    • Golgi integral membrane protein antibody
    • Golgi phosphoprotein 4 antibody
    • Golgi phosphoprotein of 130 kDa antibody
    • Golgi-localized phosphoprotein of 130 kDa antibody
    • GOLI4_HUMAN antibody
    • golim4 antibody
    • GOLPH 4 antibody
    • GPP 130 antibody
    • GPP130 antibody
    • P 138 antibody
    • P138 antibody
    • type II Golgi membrane protein antibody
    see all

Images

  • Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling GOLPH4/GPP130 with ab181849 at 1/90 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

  • All lanes : Anti-GOLPH4/GPP130 antibody [EPR13445] (ab181849) at 1/20000 dilution

    Lane 1 : Y79 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution

    Predicted band size: 82 kDa

References

ab181849 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181849.
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