Product nameAnti-GOLPH4/GPP130 antibody [EPR13445]
See all GOLPH4/GPP130 primary antibodies
DescriptionRabbit monoclonal [EPR13445] to GOLPH4/GPP130
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
within Human GOLPH4/GPP130 aa 650 to the C-terminus. The exact sequence is proprietary.
Database link: O00461
- Y79, HeLa and HepG2 cell lysates; HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181849 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 130 kDa (predicted molecular weight: 82 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionPlays a role in endosome to Golgi protein trafficking; mediates protein transport along the late endosome-bypass pathway from the early endosome to the Golgi.
Sequence similaritiesBelongs to the GOLIM4 family.
modificationsPhosphorylated probably by c-AMP-dependent kinases in its lumenal part.
O-glycosylated; modified by sialic acid residues.
N-glycosylated; N-glycans are probably of the complex type and modified by sialic acid residues.
Cellular localizationGolgi apparatus > Golgi stack membrane. Endosome membrane. Localizes to cis and medial Golgi cisternae. Probably cycles between early Golgi and distal compartments like endosome.
- Information by UniProt
- 130 kDa golgi localized phosphoprotein antibody
- cis antibody
- cis Golgi localized calcium binding protein antibody
Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling GOLPH4/GPP130 with ab181849 at 1/90 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.
All lanes : Anti-GOLPH4/GPP130 antibody [EPR13445] (ab181849) at 1/20000 dilution
Lane 1 : Y79 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution
Predicted band size: 82 kDa
ab181849 has not yet been referenced specifically in any publications.