Anti-GORASP2/GRASP55 antibody (ab74579)
Key features and details
- Rabbit polyclonal to GORASP2/GRASP55
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GORASP2/GRASP55 antibody
See all GORASP2/GRASP55 primary antibodies -
Description
Rabbit polyclonal to GORASP2/GRASP55 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human GORASP2/GRASP55 (internal sequence).
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Positive control
- HeLa, HepG2, Jurkat and LOVO cell extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab74579 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 47 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 47 kDa. |
Target
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Function
May be involved in assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA. -
Sequence similarities
Belongs to the GORASP family.
Contains 1 PDZ (DHR) domain. -
Post-translational
modificationsMyristoylated. Myristoylation is essential for the Golgi targeting.
Palmitoylated.
Phosphorylated in mitotic cells. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 26003 Human
- Entrez Gene: 70231 Mouse
- Entrez Gene: 113961 Rat
- Omim: 608693 Human
- SwissProt: Q9H8Y8 Human
- SwissProt: Q99JX3 Mouse
- SwissProt: Q9R064 Rat
- Unigene: 431317 Human
see all -
Alternative names
- AW552058 antibody
- DKFZp434D156 antibody
- FLJ13139 antibody
see all
Images
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All lanes : Anti-GORASP2/GRASP55 antibody (ab74579) at 1/500 dilution
Lane 1 : HeLa cell extracts
Lane 2 : HepG2 cell extracts
Lane 3 : Jurkat cell extracts
Lane 4 : LOVO cell extracts
Lane 5 : HeLa cell extracts with immunizing peptide at 10 µg
Lysates/proteins at 10 µg per lane.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab74579 has been referenced in 2 publications.
- Park H et al. TMED3 Complex Mediates ER Stress-Associated Secretion of CFTR, Pendrin, and SARS-CoV-2 Spike. Adv Sci (Weinh) 9:e2105320 (2022). PubMed: 35748162
- Noh SH et al. Specific autophagy and ESCRT components participate in the unconventional secretion of CFTR. Autophagy 14:1761-1778 (2018). PubMed: 29969945