Overview

  • Product name

    Anti-GORASP2/GRASP55 antibody
    See all GORASP2/GRASP55 primary antibodies
  • Description

    Mouse polyclonal to GORASP2/GRASP55
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Full length protein corresponding to Human GORASP2/GRASP55. (NP_056345.3, 1 a.a. ~ 452 a.a)

  • Positive control

    • mouse neural stem cell lysate, GORASP2/GRASP55 transfected 293T lysate.
  • General notes

    Protein previously labeled as GORASP2.

Properties

Applications

Our Abpromise guarantee covers the use of ab68713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa).

Target

  • Function

    May be involved in assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.
  • Sequence similarities

    Belongs to the GORASP family.
    Contains 1 PDZ (DHR) domain.
  • Post-translational
    modifications

    Myristoylated. Myristoylation is essential for the Golgi targeting.
    Palmitoylated.
    Phosphorylated in mitotic cells.
  • Cellular localization

    Golgi apparatus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AW552058 antibody
    • DKFZp434D156 antibody
    • FLJ13139 antibody
    • Golgi phosphoprotein 6 antibody
    • Golgi reassembly stacking protein 2 antibody
    • Golgi reassembly stacking protein 2, 55kDa antibody
    • Golgi reassembly stacking protein of 55 kDa antibody
    • Golgi reassembly-stacking protein 2 antibody
    • Golgi reassembly-stacking protein of 55 kDa antibody
    • GOLPH2 antibody
    • GOLPH6 antibody
    • GORASP2 antibody
    • GORS2_HUMAN antibody
    • GRASP55 antibody
    • GRS2 antibody
    • p59 antibody
    • RP23-428O15.1 antibody
    see all

Images

  • All lanes : Anti-GORASP2/GRASP55 antibody (ab68713) at 1/500 dilution

    Lane 1 : GORASP2 / GRASP5 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
    Lane 2 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size: 48 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?

  • Anti-GORASP2/GRASP55 antibody (ab68713) at 1/500 dilution + neural stem cell lysate at 30 µg

    Secondary
    Peroxidase labeled horse anti-mouse IgG (H+L) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?


    Exposure time: 10 minutes

    See Abreview

References

ab68713 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Neural stem cell line)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
30 µg
Specification
Neural stem cell line
Blocking step
Milk as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Ms. Melina Reisenberg

Verified customer

Submitted Feb 01 2010

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