Overview

  • Product name

    Anti-GORASP2/GRASP55 antibody - C-terminal
    See all GORASP2/GRASP55 primary antibodies
  • Description

    Rabbit polyclonal to GORASP2/GRASP55 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide within Human GORASP2/GRASP55 aa 400-450 (C terminal). The exact sequence is proprietary. (NP_056345.3).
    Sequence:

    TTAKADAASSLTVDVTPPTAKAPTTVEDRVGDSTPVSEKPVSAAVDANAS E


    Database link: Q9H8Y8

  • Positive control

    • HeLa and 293T whole cell lysates.
  • General notes

    Protein previously labeled as GORASP2.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab192910 was affinity purified using an epitope specific to GORASP2/GRASP55 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 47 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    May be involved in assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.
  • Sequence similarities

    Belongs to the GORASP family.
    Contains 1 PDZ (DHR) domain.
  • Post-translational
    modifications

    Myristoylated. Myristoylation is essential for the Golgi targeting.
    Palmitoylated.
    Phosphorylated in mitotic cells.
  • Cellular localization

    Golgi apparatus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AW552058 antibody
    • DKFZp434D156 antibody
    • FLJ13139 antibody
    • Golgi phosphoprotein 6 antibody
    • Golgi reassembly stacking protein 2 antibody
    • Golgi reassembly stacking protein 2, 55kDa antibody
    • Golgi reassembly stacking protein of 55 kDa antibody
    • Golgi reassembly-stacking protein 2 antibody
    • Golgi reassembly-stacking protein of 55 kDa antibody
    • GOLPH2 antibody
    • GOLPH6 antibody
    • GORASP2 antibody
    • GORS2_HUMAN antibody
    • GRASP55 antibody
    • GRS2 antibody
    • p59 antibody
    • RP23-428O15.1 antibody
    see all

Images

  • All lanes : Anti-GORASP2/GRASP55 antibody - C-terminal (ab192910) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 47 kDa


    Exposure time: 30 seconds


    Lysates prepared using NETN lysis buffer.

  • Detection of GORASP2/GRASP55 in immunoprecipitates of 293T whole cell lysate prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab192910 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.

    Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab192910 has not yet been referenced specifically in any publications.

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