Key features and details
- Rabbit polyclonal to GORASP2/GRASP55 - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GORASP2/GRASP55 antibody - C-terminal
See all GORASP2/GRASP55 primary antibodies
DescriptionRabbit polyclonal to GORASP2/GRASP55 - C-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
- HeLa and 293T whole cell lysates.
Protein previously labeled as GORASP2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab192910 was affinity purified using an epitope specific to GORASP2/GRASP55 immobilized on solid support.
Our Abpromise guarantee covers the use of ab192910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 47 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMay be involved in assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.
Sequence similaritiesBelongs to the GORASP family.
Contains 1 PDZ (DHR) domain.
modificationsMyristoylated. Myristoylation is essential for the Golgi targeting.
Phosphorylated in mitotic cells.
Cellular localizationGolgi apparatus membrane.
- Information by UniProt
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All lanes : Anti-GORASP2/GRASP55 antibody - C-terminal (ab192910) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 30 seconds
Lysates prepared using NETN lysis buffer.
Detection of GORASP2/GRASP55 in immunoprecipitates of 293T whole cell lysate prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab192910 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab192910 has not yet been referenced specifically in any publications.