Overview

  • Product name

    Anti-GORASP2/GRASP55 antibody [CL2610]
    See all GORASP2/GRASP55 primary antibodies
  • Description

    Mouse monoclonal [CL2610] to GORASP2/GRASP55
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human GORASP2/GRASP55 aa 380-452.
    Sequence:

    SGELLSSLPPTSNAPSDPATTTAKADAASSLTVDVTPPTAKAPTTVEDRV GDSTPVSEKPVSAAVDANASESP


    Database link: Q9H8Y8

  • Epitope

    Binds to an epitope located within the peptide sequence DAASSLTVDVTPPTA (aa 405-412) as determined by overlapping synthetic peptides.
  • Positive control

    • U251 cell line lysate; Human prostate, rectum and endometrium tissues; HeLa, A431, MCF7, U2OS and U251 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab211532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/5000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 47 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100

Target

  • Function

    May be involved in assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.
  • Sequence similarities

    Belongs to the GORASP family.
    Contains 1 PDZ (DHR) domain.
  • Post-translational
    modifications

    Myristoylated. Myristoylation is essential for the Golgi targeting.
    Palmitoylated.
    Phosphorylated in mitotic cells.
  • Cellular localization

    Golgi apparatus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AW552058 antibody
    • DKFZp434D156 antibody
    • FLJ13139 antibody
    • Golgi phosphoprotein 6 antibody
    • Golgi reassembly stacking protein 2 antibody
    • Golgi reassembly stacking protein 2, 55kDa antibody
    • Golgi reassembly stacking protein of 55 kDa antibody
    • Golgi reassembly-stacking protein 2 antibody
    • Golgi reassembly-stacking protein of 55 kDa antibody
    • GOLPH2 antibody
    • GOLPH6 antibody
    • GORASP2 antibody
    • GORS2_HUMAN antibody
    • GRASP55 antibody
    • GRS2 antibody
    • p59 antibody
    • RP23-428O15.1 antibody
    see all

Images

  • All lanes : Anti-GORASP2/GRASP55 antibody [CL2610] (ab211532)

    Lane 1 : U-251 cells, transfected with control siRNA
    Lane 2 : U-251 cells, transfected with target specific siRNA probe #1
    Lane 3 : U-251 cells, transfected with target specific siRNA probe #2

    Predicted band size: 47 kDa



    Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.

  • Immunofluorescent analysis of U251 cells (PFA-fixed/Triton X-100 permeabilized) labeling GORASP2 with ab211532 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.

  • Immunohistochemical analysis of paraffin-embedded Human rectum tissue labeling GORASP2 with ab211532 at 1/5000 dilution.

  • Anti-GORASP2/GRASP55 antibody [CL2610] (ab211532) at 1/500 dilution + U251 cell line lysate

    Predicted band size: 47 kDa

  • Immunofluorescent analysis of U2OS cells (PFA-fixed/Triton X-100 permeabilized) labeling GORASP2 with ab211532 at 4 μg/ml dilution (green). Microtubule- and nuclear probes are visualized in red and blue respectively.

  • Immunofluorescent analysis of MCF7 cells (PFA-fixed/Triton X-100 permeabilized) labeling GORASP2 with ab211532 at 4 μg/ml dilution. Microtubule- and nuclear probes are visualized in red and blue respectively.

  • Immunofluorescent analysis of A431 cells (PFA-fixed/Triton X-100 permeabilized) labeling GORASP2 with ab211532 at 4 μg/ml dilution. Microtubule- and nuclear probes are visualized in red and blue respectively.

  • Immunofluorescent analysis of HeLa cells (PFA-fixed/Triton X-100 permeabilized) labeling GORASP2 with ab211532 at 4 μg/ml dilution. Microtubule- and nuclear probes are visualized in red and blue respectively.

  • Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling GORASP2 with ab211532 at 1/5000 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling GORASP2 with ab211532 at 1/5000 dilution.

References

ab211532 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
30 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Nov 08 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
HeLa
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Nov 08 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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