Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
30 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBS
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-Ms
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 55 kDa Non-specific: 130 kDa
Positive control
Band around 130 kDa likely represents dimer
Dr. Armen Petrosyan
Verified customer
Submitted Nov 08 2019