• Product name
  • Description
    Rabbit polyclonal to GP210
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Xenopus laevis
    Predicted to work with: Mammals
  • Immunogen

    Purified bacterial N-terminal fusion protein

  • General notes
    Storage in frost-free freezers is not recommended.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituent: Whole serum
  • Purity
    Whole antiserum
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab15600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 210 kDa.
ICC/IF Use at an assay dependent dilution.


  • Function
    Nucleoporin essential for nuclear pore assembly and fusion, nuclear pore spacing, as well as structural integrity.
  • Tissue specificity
    Ubiquitous expression, with highest levels in lung, liver, pancreas, testis, and ovary, intermediate levels in brain, kidney, and spleen, and lowest levels in heart and skeletal muscle.
  • Sequence similarities
    Belongs to the NUP210 family.
  • Post-translational
    N-glycosylated, but not all potential glycosylation sites may be used. Contains high-mannose type oligosaccharides.
    Phosphorylated at Ser-1881 in mitosis specifically; not phosphorylated in interphase.
  • Cellular localization
    Nucleus > nuclear pore complex. Nucleus membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ22389 antibody
    • GP 210 antibody
    • KIAA0906 antibody
    • Nuclear envelope pore membrane protein POM 210 antibody
    • Nuclear pore membrane glycoprotein 210 antibody
    • Nuclear pore protein gp210 antibody
    • Nucleoporin 210 antibody
    • Nucleoporin 210kDa antibody
    • Nucleoporin Nup210 antibody
    • Nucleoporin210 antibody
    • NUP 210 antibody
    • Nup210 antibody
    • PO210_HUMAN antibody
    • POM 210 antibody
    • POM210 antibody
    • Pore membrane protein of 210 kDa antibody
    see all


  • HeLa cells were permeabilised in PBS Triton X-100 (0.2%), fixed in 3.7% formaldehyde and blocked in 2% milk in PBS-T. The slides were incubated with 1/1000 dilution of ab15600 for 1 hour at room temperature. The slides were washed 3 times prior to incubation with the secondary antibody. ab15600 clearly stains the nuclear envelope (red). The DNA is stained with DAPI (blue).


This product has been referenced in:
  • Fernández-Medarde A  et al. Laser microdissection and microarray analysis of the hippocampus of Ras-GRF1 knockout mice reveals gene expression changes affecting signal transduction pathways related to memory and learning. Neuroscience 146:272-85 (2007). Read more (PubMed: 17321057) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
African Green Monkey Cell (Kidney epithelial cell (Vero))
Kidney epithelial cell (Vero)
Yes - 0.2% triton x-100

Eric Alonas

Verified customer

Submitted Nov 18 2014


Thank you very much for contacting us with your question.

We only have full size vials of all of our products, so unfortunately we do not have any smalltrialvials to send out. Do you have the Arabidopsis amino acid sequence for GP210? I could not find it myself, but I would be happy to run an alignment to see if we would predict ab15600 to cross-react with your samples.

We do have a testing program available to try an antibody in an untested species. As part of this program, you would initially purchase ab15600 at full price, test it with Arabidopsis samples, and then submit an online Abreview with your results. In exchange, we will give you a code that can be used for 1 free primary antibody on a future order. I can send more details about this program if you would like.

I hope that this information will be useful, but if there is anything else that we can do for you, please let me know and I'll be happy to help.

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Thank you for the details that you have provided and for your patience. Did you also try this antibody in Western blotting? I saw a note from a colleague who spoke to you and both IHC and WB are mentioned as applications that you have tried. If yes, can you please provide some details regarding your protocol? Also, do you have the Abcam order number or purchase order number that was used when you purchased this antibody? Thanks again, and we look forward to hearing from you.

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Thank you for contacting us. Please find links to the forms I discussed with you (one for IHC, one for Western blot). Once you have submitted these forms, we will take a look at your protocol and try to make any suggestions we think will help improve your results. Western Blot: https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=15600&mode=questionaire IHC: https://www.abcam.com/index.html?section=ihc&pageconfig=technical&intAbID=15600&mode=questionaire I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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