Overview

  • Product name

    Anti-GP210 antibody
  • Description

    Rabbit polyclonal to GP210
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Monkey
    Predicted to work with: Mammals
  • Immunogen

    Fusion protein (Rat) (C terminal).

  • Positive control

    • rat liver
  • General notes

    Storage in frost-free freezers is not recommended.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: Whole serum
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB 1/1000. Predicted molecular weight: 210 kDa.

Target

  • Function

    Nucleoporin essential for nuclear pore assembly and fusion, nuclear pore spacing, as well as structural integrity.
  • Tissue specificity

    Ubiquitous expression, with highest levels in lung, liver, pancreas, testis, and ovary, intermediate levels in brain, kidney, and spleen, and lowest levels in heart and skeletal muscle.
  • Sequence similarities

    Belongs to the NUP210 family.
  • Post-translational
    modifications

    N-glycosylated, but not all potential glycosylation sites may be used. Contains high-mannose type oligosaccharides.
    Phosphorylated at Ser-1881 in mitosis specifically; not phosphorylated in interphase.
  • Cellular localization

    Nucleus > nuclear pore complex. Nucleus membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ22389 antibody
    • GP 210 antibody
    • KIAA0906 antibody
    • Nuclear envelope pore membrane protein POM 210 antibody
    • Nuclear pore membrane glycoprotein 210 antibody
    • Nuclear pore protein gp210 antibody
    • Nucleoporin 210 antibody
    • Nucleoporin 210kDa antibody
    • Nucleoporin Nup210 antibody
    • Nucleoporin210 antibody
    • NUP 210 antibody
    • Nup210 antibody
    • PO210_HUMAN antibody
    • POM 210 antibody
    • POM210 antibody
    • Pore membrane protein of 210 kDa antibody
    see all

Images

  • ab15601 used in Western blot at a 1/1000 with rat liver nuclear envelopes.
  • HeLa cells grown on coverslips were washed twice with PBS and permeabilized with 0.2% Triton X-100 in PBS for 2 min at room temperature.  Following 2 PBS washes, the coverslips were fixed with 3.7% formaldehyde and subsequently blocked in 2% skim milk powder in PBS-T (PBS containing 0.1% Tween 20) for 2 hrs.  For detection of gp210, ab15601 was diluted 1:500-1:1000 in 2% skim milk powder in PBS-T and incubated on the coverslips for 1 hour at RT.  Following incubation with primary antibodies, coverslips were washed three times with PBS-T over a period of 45 min.  Antibody binding was detected with donkey anti-rabbit antibodies conjugated to Cy3 at a dilution of 1:2500.

References

This product has been referenced in:

  • Jeong K  et al. Rab6-mediated retrograde transport regulates inner nuclear membrane targeting of caveolin-2 in response to insulin. Traffic 13:1218-33 (2012). Read more (PubMed: 22607032) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

- What is PBT?
- Did you try no primary control?
- Have you done any sort of troubleshooting?
- Could you provide the order number?
- How the antibody was stored?

Thank you very much for your cooperation. I will look forward to hearing from you soon.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up