Overview

  • Product name
  • Description
    Rabbit polyclonal to GP210
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Monkey
    Predicted to work with: Mammals
  • Immunogen

    Fusion protein (Rat) (C terminal).

  • Positive control
    • rat liver
  • General notes
    Storage in frost-free freezers is not recommended.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituent: Whole serum
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB 1/1000. Predicted molecular weight: 210 kDa.

Target

  • Function
    Nucleoporin essential for nuclear pore assembly and fusion, nuclear pore spacing, as well as structural integrity.
  • Tissue specificity
    Ubiquitous expression, with highest levels in lung, liver, pancreas, testis, and ovary, intermediate levels in brain, kidney, and spleen, and lowest levels in heart and skeletal muscle.
  • Sequence similarities
    Belongs to the NUP210 family.
  • Post-translational
    modifications
    N-glycosylated, but not all potential glycosylation sites may be used. Contains high-mannose type oligosaccharides.
    Phosphorylated at Ser-1881 in mitosis specifically; not phosphorylated in interphase.
  • Cellular localization
    Nucleus > nuclear pore complex. Nucleus membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ22389 antibody
    • GP 210 antibody
    • KIAA0906 antibody
    • Nuclear envelope pore membrane protein POM 210 antibody
    • Nuclear pore membrane glycoprotein 210 antibody
    • Nuclear pore protein gp210 antibody
    • Nucleoporin 210 antibody
    • Nucleoporin 210kDa antibody
    • Nucleoporin Nup210 antibody
    • Nucleoporin210 antibody
    • NUP 210 antibody
    • Nup210 antibody
    • PO210_HUMAN antibody
    • POM 210 antibody
    • POM210 antibody
    • Pore membrane protein of 210 kDa antibody
    see all

Images

  • ab15601 used in Western blot at a 1/1000 with rat liver nuclear envelopes.
  • HeLa cells grown on coverslips were washed twice with PBS and permeabilized with 0.2% Triton X-100 in PBS for 2 min at room temperature.  Following 2 PBS washes, the coverslips were fixed with 3.7% formaldehyde and subsequently blocked in 2% skim milk powder in PBS-T (PBS containing 0.1% Tween 20) for 2 hrs.  For detection of gp210, ab15601 was diluted 1:500-1:1000 in 2% skim milk powder in PBS-T and incubated on the coverslips for 1 hour at RT.  Following incubation with primary antibodies, coverslips were washed three times with PBS-T over a period of 45 min.  Antibody binding was detected with donkey anti-rabbit antibodies conjugated to Cy3 at a dilution of 1:2500.

References

ab15601 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Question
Answer

Thank you for contacting us.

Your credit note ID is XXXXX.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

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Question
Answer

Unfortunately this order number does not match the order number we have in or system for antibody ab15601. Could you please double check with purchasing department?

Thanks!

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Answer

Unfortunately, both antibodies are form same collaborator; this however does not mean both are faulty. If you would like to try one then I can send it to you as replacement, else I will be happy to provide refund.

IN order to receive refund or replacement could you please provide the purchase order number?

Thanks!

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Answer

Thank you for contacting us.

I have been in contact with the originator of this antibody. They have taken longer time than expected. I am sorry for the delay. In order to avoid further delay I am happy to provide you a replacement antibody, could you please choose any other anti GP210 ab from catalogue.

Many thanks for your cooperation in this case.

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Answer

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

- What is PBT?
- Did you try no primary control?
- Have you done any sort of troubleshooting?
- Could you provide the order number?
- How the antibody was stored?

Thank you very much for your cooperation. I will look forward to hearing from you soon.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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