Product nameAnti-gp78 antibody
See all gp78 primary antibodies
DescriptionRabbit polyclonal to gp78
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
Synthetic peptide corresponding to Human gp78 aa 500-550.
Database link: NP_001135.3
- HeLa and 293T whole cell lysates
Previously labelled as AMFR.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab101284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 73 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionE3 ubiquitin-protein ligase which can target both itself and other proteins including CD3D and APOB for proteasomal degradation. Mediates polyubiquitination of CYP3A4. Specific receptor for the autocrine motility factor. Mediates tumor invasion and metastasis. Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Promotes ubiquitination of misfolded proteins such as mutant CFTR; proposed to mediate sequential ubiquitination by recognizing already ubiquitin-conjugated substrates and to cooperate with E3 ubiquitin-protein ligase RNF5.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 CUE domain.
Contains 1 RING-type zinc finger.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- AMF receptor antibody
- AMF receptor isoform 1 antibody
- AMF receptor isoform 2 antibody
All lanes : Anti-gp78 antibody (ab101284) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 73 kDa
Exposure time: 3 minutes
Detection of Human gp78 in immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab101284 at 3 µg/mg lysate for immunoprecipitation and at 1 µg/ml for subsequent Western blotting.
Detection by chemiluminescence with an exposure time of 30 seconds.
This product has been referenced in:
- Ramachandran S et al. SYVN1, NEDD8, and FBXO2 Proteins Regulate ?F508 Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Ubiquitin-mediated Proteasomal Degradation. J Biol Chem 291:25489-25504 (2016). Read more (PubMed: 27756846) »