Key features and details
- Rabbit polyclonal to GPA33
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GPA33 antibody
See all GPA33 primary antibodies
DescriptionRabbit polyclonal to GPA33
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Fusion protein within Human GPA33 (internal sequence). The exact sequence is proprietary. (BC069723). The identity of the protein fusion partner is GST.
Database link: Q99795
- 293T, Human colon cancer tissue and 231 lysates; Human colon cancer tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab197370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 36 kDa.|
|IHC-P||1/50 - 1/200.|
FunctionMay play a role in cell-cell recognition and signaling.
Tissue specificityExpressed in normal gastrointestinal epithelium and in 95% of colon cancers.
Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
modificationsN-glycosylated, contains approximately 8 kDa of N-linked carbohydrate.
- Information by UniProt
- A33 antibody
- Cell surface A33 antigen antibody
- Cell surface A33 antigen precursor antibody
All lanes : Anti-GPA33 antibody (ab197370) at 1/600 dilution
Lane 1 : 293T cell lysate
Lane 2 : Human colon cancer tissue lysate
Lane 3 : 231 cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 36 kDa
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling GPA33 with ab197370 at 1/30 dilution.
ab197370 has not yet been referenced specifically in any publications.