Anti-GPA33 antibody (ab197370)

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Overview

  • Product name

    Anti-GPA33 antibody
    See all GPA33 primary antibodies

  • Description

    Rabbit polyclonal to GPA33

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Fusion protein within Human GPA33 (internal sequence). The exact sequence is proprietary. (BC069723). The identity of the protein fusion partner is GST.
    Database link: Q99795

  • Positive control

    • 293T, Human colon cancer tissue and 231 lysates; Human colon cancer tissue.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab197370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 36 kDa.
IHC-P 1/50 - 1/200.

Target

  • Function

    May play a role in cell-cell recognition and signaling.

  • Tissue specificity

    Expressed in normal gastrointestinal epithelium and in 95% of colon cancers.

  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Post-translational
    modifications

    N-glycosylated, contains approximately 8 kDa of N-linked carbohydrate.
    Palmitoylated.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • A33 antibody
      • Cell surface A33 antigen antibody
      • Cell surface A33 antigen precursor antibody
      • Glycoprotein A33 (transmembrane) antibody
      • Glycoprotein A33 antibody
      • Gpa33 antibody
      • GPA33_HUMAN antibody
      • MGC129986 antibody
      • MGC129987 antibody
      • OTTHUMP00000032346 antibody
      • Transmembrane glycoprotein A33 antibody
      see all

    Images

    • Western blot - Anti-GPA33 antibody (ab197370)
      Western blot - Anti-GPA33 antibody (ab197370)
      All lanes : Anti-GPA33 antibody (ab197370) at 1/600 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Human colon cancer tissue lysate
      Lane 3 : 231 cell lysate

      Lysates/proteins at 40 µg per lane.

      Predicted band size: 36 kDa



      15% SDS-PAGE.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody (ab197370)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody (ab197370)

      Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling GPA33 with ab197370 at 1/30 dilution.

    References

    ab197370 has not yet been referenced specifically in any publications.

    Publishing research using ab197370? Please let us know so that we can cite the reference in this datasheet.

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