Anti-GPA33 antibody (ab197370)
Key features and details
- Rabbit polyclonal to GPA33
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GPA33 antibody
See all GPA33 primary antibodies -
Description
Rabbit polyclonal to GPA33 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Fusion protein within Human GPA33 (internal sequence). The exact sequence is proprietary. (BC069723). The identity of the protein fusion partner is GST.
Database link: Q99795 -
Positive control
- 293T, Human colon cancer tissue and 231 lysates; Human colon cancer tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab197370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2000. Predicted molecular weight: 36 kDa. | |
| IHC-P | 1/50 - 1/200. |
Target
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Function
May play a role in cell-cell recognition and signaling. -
Tissue specificity
Expressed in normal gastrointestinal epithelium and in 95% of colon cancers. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsN-glycosylated, contains approximately 8 kDa of N-linked carbohydrate.
Palmitoylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10223 Human
- Omim: 602171 Human
- SwissProt: Q99795 Human
- Unigene: 651244 Human
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Alternative names
- A33 antibody
- Cell surface A33 antigen antibody
- Cell surface A33 antigen precursor antibody
see all
Images
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Western blot - Anti-GPA33 antibody (ab197370)All lanes : Anti-GPA33 antibody (ab197370) at 1/600 dilution
Lane 1 : 293T cell lysate
Lane 2 : Human colon cancer tissue lysate
Lane 3 : 231 cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 36 kDa15% SDS-PAGE.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPA33 antibody (ab197370)Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling GPA33 with ab197370 at 1/30 dilution.
Protocols
Datasheets and documents
References (0)
ab197370 has not yet been referenced specifically in any publications.
