GPAM overexpression 293T lysate (whole cell) (ab156677)
- Datasheet
- References
- Protocols
Overview
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Product nameGPAM overexpression 293T lysate (whole cell)
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General notes
ab156677 is a 293T cell transfected lysate in which Human GPAM has been transiently over-expressed using a pCMV-GPAM plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
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Tested applicationsSuitable for: WBmore details
Properties
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FormLiquid
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Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
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Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% SDS, 0.788% Tris HCl, 10% Glycerol -
Concentration information loading... -
Purity>95% by SDS-PAGE
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Research areas
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BackgroundFunction: Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Pathway: Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. Similarity: Belongs to the GPAT/DAPAT family. Domain: The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
Applications
Our Abpromise guarantee covers the use of ab156677 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. |
References
ab156677 has not yet been referenced specifically in any publications.
