Key features and details
- Rabbit polyclonal to GPAT4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GPAT4 antibody
See all GPAT4 primary antibodies
DescriptionRabbit polyclonal to GPAT4
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Orangutan
Synthetic peptide corresponding to Human GPAT4 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in human skeletal muscle tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
Our Abpromise guarantee covers the use of ab76707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionEsterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Active against both saturated and unsaturated long-chain fatty acyl-CoAs.
Tissue specificityUbiquitous. Relatively high level of expression in skeletal muscle, heart and testis. Relatively low level of expression in lung.
PathwayPhospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
Sequence similaritiesBelongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
DomainThe HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- 1 acyl sn glycerol 3 phosphate acyltransferase zeta antibody
- 1 AGP acyltransferase 6 antibody
- 1 AGPAT 6 antibody
Anti-GPAT4 antibody (ab76707) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
The band observed at 49 kDa could potentially be a cleaved form of Human 1-acyl-sn-glycerol-3-phosphate acyltransferase zeta precursor (AGPAT6). The protein contains a 36 amino acid signal peptide, which when cleaved gives an expected molecular weight of 48 kDa.
ab76707 has been referenced in 1 publication.
- Sadh K et al. Feeding-fasting dependent recruitment of membrane microdomain proteins to lipid droplets purified from the liver. PLoS One 12:e0183022 (2017). PubMed: 28800633