Overview

  • Product name

  • Description

    Rabbit polyclonal to GPAT4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human GPAT4 aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab91636)

  • Positive control

    • This antibody gave a positive signal in human skeletal muscle tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab76707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Active against both saturated and unsaturated long-chain fatty acyl-CoAs.
    • Tissue specificity

      Ubiquitous. Relatively high level of expression in skeletal muscle, heart and testis. Relatively low level of expression in lung.
    • Pathway

      Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
    • Sequence similarities

      Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
    • Domain

      The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
    • Cellular localization

      Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 1 acyl sn glycerol 3 phosphate acyltransferase zeta antibody
      • 1 AGP acyltransferase 6 antibody
      • 1 AGPAT 6 antibody
      • 1-acylglycerol-3-phosphate O-acyltransferase 6 antibody
      • 1-AGP acyltransferase 6 antibody
      • 1-AGPAT 6 antibody
      • Acyl-CoA:glycerol-3-phosphate acyltransferase 4 antibody
      • AGPAT6 antibody
      • Glycerol-3-phosphate acyltransferase 4 antibody
      • GPAT4 antibody
      • GPAT4_HUMAN antibody
      • LPAAT zeta antibody
      • LPAAT-zeta antibody
      • Lysophosphatidic acid acyltransferase zeta antibody
      see all

    Images

    • Anti-GPAT4 antibody (ab76707) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 52 kDa
      Observed band size: 49 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 10 minutes


      The band observed at 49 kDa could potentially be a cleaved form of Human 1-acyl-sn-glycerol-3-phosphate acyltransferase zeta precursor (AGPAT6). The protein contains a 36 amino acid signal peptide, which when cleaved gives an expected molecular weight of 48 kDa.

    References

    This product has been referenced in:

    • Sadh K  et al. Feeding-fasting dependent recruitment of membrane microdomain proteins to lipid droplets purified from the liver. PLoS One 12:e0183022 (2017). Read more (PubMed: 28800633) »
    See 1 Publication for this product

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