Overview

  • Product name

    Anti-GPATCH8 antibody
  • Description

    Rabbit polyclonal to GPATCH8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence: SIASVFKDHA EEGTSEDGTK PDEKSSDQGL QKVGDSDGSS NLDGKKEDED PQDGGSLAST LSKLKRMKRE EGAGATEPEY YHYIPPAHCK VKPN, corresponding to amino acids of Human GPATCH8.

  • Positive control

    • Human rectum tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

Target

Images

  • Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus & mitochondria.
  • ab126300 at 1/200 dilution, staining GPATCH8 in paraffin-embedded Human rectum tissue, by Immunohistochemistry.

References

ab126300 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (6% gel)
Sample
Mouse Cell lysate - whole cell (mouse ES cells)
Specification
mouse ES cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Nov 11 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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