Overview

  • Product name
  • Description
    Rabbit polyclonal to GPBB
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 519-813 of Human GPBB (UniProt P11216).

  • Positive control
    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4, Raji and Mouse brain lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab154969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 96 kDa.

Target

  • Function
    Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
  • Sequence similarities
    Belongs to the glycogen phosphorylase family.
  • Post-translational
    modifications
    Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain glycogen phosphorylase antibody
    • Glycogen phosphorylase B antibody
    • Glycogen phosphorylase brain form antibody
    • Glycogen Phosphorylase Isoenzyme BB antibody
    • Glycogen phosphorylase, brain form antibody
    • MGC9213 antibody
    • Phosphorylase glycogen brain antibody
    • PYGB antibody
    • PYGB_HUMAN antibody
    see all

Images

  • Anti-GPBB antibody (ab154969) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 96 kDa



    7.5% SDS PAGE
  • Anti-GPBB antibody (ab154969) at 1/2000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 96 kDa



    7.5% SDS PAGE

References

This product has been referenced in:
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (gel 10% SDS-PAGE)
Sample
Mouse Tissue lysate - whole (Brain)
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Karine Thibault

Verified customer

Submitted Nov 05 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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